NDLI: Self-renewal of the human gastric epithelium: new insights from expression profiling using laser microdissection.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Kouznetsova, Irina Kalinski, Thomas Meyer, Frank Hoffmann, Werner
Description	Country affiliation: Germany Author Affiliation: Kouznetsova I ( Institute of Molecular Biology and Medicinal Chemistry, Otto-von-Guericke University, Leipziger Str 44, D-39120 Magdeburg, Germany.)
Abstract	The gastric mucosa is subject to continual bidirectional renewal by differentiation from stem and transit amplifying cells. It was the aim of this study to characterize the self-renewal of the human gastric mucosa and its two major types of glands in the fundus and antrum, respectively. Three characteristic regions (pit, proliferative, and lower neck regions) were isolated from fundic and antral units by the use of laser microdissection, and expression profiles concerning 15 marker genes were generated by RT-PCR analysis. The surface mucous cells (SMCs) of fundic and antral units differed in their expression of at least four secretory genes, i.e., gastric lipase, TFF3, FCGBP, and lysozyme. The maturation of mucous neck cells was shown to occur stepwise, first towards a mucous phenotype followed by a serous differentiation step. Also, a stepwise maturation of both the antral SMCs and antral gland cells was observed. Additionally, the presence of gastric lipase was also demonstrated for the first time in antral gland cells. In conclusion, the different expression profiles of SMCs of the fundic and antral units could be the basis for the different self-renewal rates of fundic and antral SMCs and could influence the spatial organization of the bacterial microbiota within the various parts of the gastric mucosa.
File Format	HTM / HTML
ISSN	1742206X
Issue Number	4
Volume Number	7
e-ISSN	17422051
Journal	Molecular BioSystems
Language	English
Publisher	Royal Society of Chemistry
Publisher Date	2011-04-01
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Discipline Molecular Biology Discipline Biochemistry Gastric Mucosa Growth & Development Metabolism Gene Expression Profiling Microdissection Adult Aged Cell Adhesion Molecules Epithelial Cells Gastric Fundus Enzymology Pathology Gene Expression Regulation Humans Lipase Middle Aged Peptides Pyloric Antrum Reverse Transcriptase Polymerase Chain Reaction Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biotechnology

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