NDLI: Synthetic lethal interactions in yeast reveal functional roles of J protein co-chaperones.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Gillies, Anne T. Taylor, Rebecca Gestwicki, Jason E.
Description	Country affiliation: United States Author Affiliation: Gillies AT ( Departments of Pathology and Biological Chemistry and the Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109-2216, USA.)
Abstract	J proteins are a diverse family of co-chaperones that cooperate with heat shock protein 70 (Hsp70) to coordinate protein quality control, especially in response to cellular stress. Current models suggest that individual J proteins might play roles in recruiting Hsp70s to specific functions, such as maintaining cell wall integrity or promoting ribosome biogenesis. However, relatively few stresses have been used to test this model and, as a result, only a few specific activities have been identified. To expand our understanding of the J protein network, we used a synthetic lethal approach in which 11 Saccharomyces cerevisiae deletion strains were treated with 12 well-characterized chemical inhibitors. The results defined new roles for specific J proteins in major signaling pathways. For example, an important role for Swa2 in cell wall integrity was identified and activities of the under-explored Jjj1, Apj1, Jjj3 and Caj1 proteins were suggested. More generally, these findings support a model in which some J proteins, such as Ydj1 and Zuo1, play 'generalist' roles, while others, such as Apj1 and Jjj2, are 'specialists', having roles in relatively few pathways. Together, these results provide new insight into the network of J proteins.
File Format	HTM / HTML
ISSN	1742206X
e-ISSN	17422051
DOI	10.1039/c2mb25248a
Journal	Molecular BioSystems
Issue Number	11
Volume Number	8
Language	English
Publisher	Royal Society of Chemistry
Publisher Date	2012-11-01
Publisher Place	Great Britain (UK)
Access Restriction	Open
Subject Keyword	Discipline Molecular Biology Discipline Biochemistry Molecular Chaperones Metabolism Saccharomyces Cerevisiae Proteins Saccharomyces Cerevisiae Calmodulin-binding Proteins Genetics Hsp40 Heat-shock Proteins Hsp70 Heat-shock Proteins Phosphoproteins Signal Transduction Vesicular Transport Proteins Research Support, N.i.h., Extramural Research Support, U.s. Gov't, Non-p.h.s.
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biotechnology

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The specialized cytosolic J-protein, Jjj1, functions in 60S ribosomal subunit biogenesis.

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Synthetic lethal interactions in yeast reveal functional roles of J protein co-chaperones

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Synthetic lethal interactions in yeast reveal functional roles of J protein co-chaperones.

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