NDLI: Structural modeling and molecular dynamics studies on the human LMTK3 domain and the mechanism of ATP binding.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Anbarasu, K. Jayanthi, S.
Description	Country affiliation: India Author Affiliation: Anbarasu K ( School of Bio Sciences and Technology, VIT University, Vellore, Tamil Nadu 632 014, India. jays66@gmail.com.)
Abstract	Estrogen positive breast cancer is a dreadful disease in women worldwide. The human estrogen receptor- (ER ) pathway plays a critical role in estrogenic signaling and targeting ER in breast cancer treatment. The key role of Lemur tyrosine kinase-3 (LMTK3) in regulation of ER has been identified and it is found to be a novel therapeutic target for breast cancer. With lack of structural studies on LMTK3, the breast cancer therapeutics research remains elusive. In this computational study, we performed structural studies on LMTK3 by structural modeling and molecular dynamics (MD) simulations of the apo state and the ATP bound state. The structure of the LMTK3 domain was developed by using I-TASSER server and validated by quality index and Ramachandran plot. MD simulation analysis explained the structural behavior of the LMTK3 domain in the dynamic system and the apo state showed defined protein folding with stable conformation. The mechanism of ATP binding was studied using molecular docking, resulting in the identification of critical residues and the ATP binding cavity. Furthermore, MD simulation of the LMTK3-ATP complex was performed and the trajectory analyses confirmed the stability and effective binding of ATP in the dynamic system. Overall, our computational reports provide more information on the structure-function relationship of LMTK3 with ATP. The critical residues Tyr185 and Asp284 found in the ATP binding cavity may be useful in designing potential inhibitors on human LMTK3.
File Format	HTM / HTML
ISSN	1742206X
Issue Number	5
Volume Number	10
e-ISSN	17422051
Journal	Molecular BioSystems
Language	English
Publisher	Royal Society of Chemistry
Publisher Date	2014-05-01
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Discipline Molecular Biology Discipline Biochemistry Membrane Proteins Chemistry Molecular Dynamics Simulation Protein-serine-threonine Kinases Adenosine Triphosphate Metabolism Apoproteins Female Humans Molecular Docking Simulation Principal Component Analysis Protein Binding Protein Structure, Tertiary Quality Control Reproducibility Of Results Time Factors Water Journal Article
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biotechnology

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