NDLI: NMR- and MS-based metabolomics: various organ responses following naphthalene intervention.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Ling, Yee Soon Liang, Hao-Jan Chung, Meng-Hsuan Lin, Ming-Huan Lin, Ching-Yu
Description	Country affiliation: Taiwan Author Affiliation: Ling YS ( Graduate Institute of Toxicology, College of Medicine, National Taiwan University, No.1, Sec. 1, Jen-ai Rd., Taipei 100, Taiwan. chingyulin@ntu.edu.tw.)
Abstract	Naphthalene, a polycyclic aromatic hydrocarbon, is a ubiquitous environmental pollutant capable of causing illness. In this study, we deconvoluted the metabolites related to naphthalene intervention in various organs by using nuclear magnetic resonance (NMR) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). Male ICR mice were intraperitoneally dosed with olive oil (vehicle), and a low dose and a high dose (100 and 200 mg kg(-1) body wt, respectively) of naphthalene. After 48 h, the lungs, liver, and kidneys were collected for analysing the metabolic responses. The metabolites were extracted and non-targeted profiled using NMR. Low NMR resolution limited the identification of the hydrophobic metabolites. Therefore, LC-MS/MS-based focus lipidomics was applied to profile phosphorylcholine-containing lipids and sphingolipids. Chemometric analysis revealed that succinate and lactate were significantly increased in the lungs, suggesting that energy metabolisms and antioxidation were increased following naphthalene treatment. In the liver, anti-oxidative stress-related metabolites increased, enabling the oxidative stress during naphthalene biotransformation and detoxification to be overcome. The elevation of glutathione protected kidneys from reactive-naphthalene-metabolite-induced injury. Significant alteration of hydrophobic metabolites (membrane constituents) revealed lung and liver were the target organs of naphthalene treatment. MS data demonstrated that phosphatidylcholine (PC) and ceramide species were significantly altered in the lungs and liver, whereas only PC was observed in the kidneys. Elevated numbers of unsaturated bonds and fatty acyl chains in both ceramides and PCs were determined to reduce cellular membrane rigidity and facilitating the trafficking of recovery elements into the cell for rejuvenation. To conclude, the complementary results of NMR- and MS-based metabolomics enabled the characterization of naphthalene-induced changes in various organs.
File Format	HTM / HTML
ISSN	1742206X
Issue Number	7
Volume Number	10
e-ISSN	17422051
Journal	Molecular BioSystems
Language	English
Publisher	Royal Society of Chemistry
Publisher Date	2014-07-01
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Discipline Molecular Biology Discipline Biochemistry Kidney Chemistry Liver Lung Metabolomics Methods Naphthalenes Toxicity Animals Chromatography, Liquid Energy Metabolism Drug Effects Magnetic Resonance Spectroscopy Male Mice Mice, Inbred Icr Administration & Dosage Oxidation-reduction Tandem Mass Spectrometry Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biotechnology

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