NDLI: Mapping the minimal regions within the ORF73 protein required for herpesvirus saimiri episomal persistence.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Griffiths, Rhoswyn Harrison, Sally M. Macnab, Stuart Whitehouse, Adrian
Description	Country affiliation: United kingdom Author Affiliation: Griffiths R ( Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK.)
Abstract	Herpesvirus saimiri (HVS) establishes a persistent infection in which the viral genome persists as a circular non-integrated episome. ORF73 tethers HVS episomes to host mitotic chromosomes, allowing episomal persistence via an interaction with the chromosome-associated protein, MeCP2. Here we demonstrate that ORF73 also interacts with the linker histone H1 via its C terminus, suggesting it associates with multiple chromosome-associated proteins. In addition, we show that the C terminus is also required for the ability of ORF73 to bind the terminal repeat region of the HVS genome. These results suggest that the ORF73 C terminus contains all the necessary elements required for HVS episomal persistence. Using a range of ORF73 C terminus deletions to rescue the episomal maintenance properties of a HVSDelta73 recombinant virus, we show that a C terminus region comprising residues 285-407 is sufficient to maintain the HVS episome in a dividing cell population.
File Format	HTM / HTML
ISSN	00221317
Issue Number	Pt 11
Volume Number	89
e-ISSN	14652099
Journal	Journal of General Virology
Language	English
Publisher	Microbiology Society
Publisher Date	2008-11-01
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Cell Line Research Support, Non-u.s. Gov't Peptide Fragments Discipline Virology Herpesvirus 2, Saimiriine Humans Saimiri Viral Proteins Metabolism Journal Article Nuclear Proteins Virology Chemistry Animals Transfection Antigens, Viral Virus Replication Cell Division Genetics Genes, Reporter Carrier Proteins
Content Type	Text
Resource Type	Article
Subject	Virology

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