NDLI: Immunogenic compositions assembled from tobacco mosaic virus-generated spherical particle platforms and foreign antigens.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Karpova, Olga Nikitin, Nikolai Chirkov, Sergey Trifonova, Ekaterina Sheveleva, Anna Lazareva, Ekaterina Atabekov, Joseph
Description	Country affiliation: RUSSIA Author Affiliation: Karpova O ( Department of Virology, Moscow State University, Moscow 119991, Russia.)
Abstract	We reported recently that RNA-free spherical particles (SPs) generated by thermal remodelling of tobacco mosaic virus (TMV) are capable of binding GFP to their surface. Here, we show that SPs represent a universal particle platform that can form compositions by binding a diversity of various foreign proteins/epitopes of viral and non-viral origin to their surface. Numerous molecules of a foreign protein linked to the SP surface were revealed by immunogold electron microscopy. Several SP-based compositions were obtained containing one of the following foreign antigens: antigenic determinant A of rubella virus E1 glycoprotein; a recombinant protein containing the M2e epitope of influenza virus A protein M2; a recombinant antigen consisting of three epitopes of influenza virus A haemagglutinin; potato virus X (PVX) coat protein (CP); BSA; and PVX CP fused with the epitope of plum pox virus CP. The 'mixed' compositions could be also assembled by binding two different foreign antigens to each of the SPs. Immunogenicity of foreign antigens adsorbed or linked covalently to SPs in the SP-based compositions was examined. The antigenic specificity of foreign antigens was retained, whereas their immunogenicity increased significantly. It was inferred that SPs exhibit immunopotentiating activity, in particular in the form of compositions comprising SP and foreign antigen linked covalently to their surface by formaldehyde.
File Format	HTM / HTML
ISSN	00221317
Issue Number	Pt 2
Volume Number	93
e-ISSN	14652099
Journal	Journal of General Virology
Language	English
Publisher	Microbiology Society
Publisher Date	2012-02-01
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Immunohistochemistry Chemistry Immunology Antigens, Viral Virion Discipline Virology Protein Binding Metabolism Tobacco Mosaic Virus Microscopy, Immunoelectron Journal Article Drug Carriers
Content Type	Text
Resource Type	Article
Subject	Virology

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