NDLI: A bi-cistronic, reporter-encoding bovine viral diarrhea virus applied in a new, effective diagnostic test.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Gebauer, Mandy Behrens, Martina König, Matthias Behrens, Sven-Erik
Description	Country affiliation: Germany Author Affiliation: Gebauer M ( Institute of Biochemistry and Biotechnology, Faculty of Life Sciences (NFI), Martin Luther University Halle-Wittenberg, Halle, Germany.); Behrens M ( Institute of Biochemistry and Biotechnology, Faculty of Life Sciences (NFI), Martin Luther University Halle-Wittenberg, Halle, Germany.); König M ( Institue of Virology, Faculty of Veterinary Medicine, Justus-Liebig-University Giessen, Giessen, Germany.); Behrens SE ( Institute of Biochemistry and Biotechnology, Faculty of Life Sciences (NFI), Martin Luther University Halle-Wittenberg, Halle, Germany sven.behrens@biochemtech.uni-halle.de.)
Abstract	Infections with bovine viral diarrhea virus (BVDV) have a huge economic impact on cattle production and reproduction worldwide. A key factor for BVDV surveillance and eventual eradication is to efficiently detect infections and to monitor herd immunity. In this study, we generated a stable, bi-cistronic BVDV that encoded EGFP in addition to the viral proteins. Applying this recombinant virus, a new flow-cytometry-based virus neutralization test was established that enabled accurate and reliable detection of field-virus-infected and vaccinated animals. The test, which is simple and fast, is expected to support novel, effective screening procedures in eradication and vaccination programmes.
File Format	HTM / HTML
ISSN	00221317
Issue Number	Pt 7
Volume Number	95
e-ISSN	14652099
Journal	Journal of General Virology
Language	English
Publisher	Microbiology Society
Publisher Date	2014-07-01
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Antibodies, Neutralizing Green Fluorescent Proteins Research Support, Non-u.s. Gov't Diagnosis, Differential Diarrhea Virus 1, Bovine Viral Discipline Virology Neutralization Tests Veterinary Medicine Antibodies, Viral Blood Journal Article Virology Diagnostic Tests, Routine Animals Immunology Flow Cytometry Mass Screening Cattle Analysis Genetics Diagnosis Bovine Virus Diarrhea-mucosal Disease Methods Genes, Reporter
Content Type	Text
Resource Type	Article
Subject	Virology

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