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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Silva, Sandro P. Dilcher, Meik Weber, Franziska Hufert, Frank T. Weidmann, Manfred Cardoso, Jedson F. Carvalho, Valéria L. Chiang, Jannifer O. Martins, Lívia C. Lima, Clayton P. S. Da Silva, Daisy E. A. Vianez-Júnior, João L. S. G. Popov, Vsevolod L. Travassos da Rosa, Amélia P. A. Tesh, Robert B. Vasconcelos, Pedro F. C. Nunes, Márcio R. T. |
| Spatial Coverage | Brazil |
| Description | Author Affiliation: Silva SP ( Center for Technological Innovation of Evandro Chagas Institute, Ministry of Health, BR 316, Km 07, s/n CEP 67.030-000, Ananindeua, Brazil spatroca@gmail.com marcionunes@iec.pa.gov.br.); Dilcher M ( Department of Virology, University Medical Center Göttingen, Kreuzbergring 57, D-37075 Göttingen, Germany.); Weber F ( Department of Virology, University Medical Center Göttingen, Kreuzbergring 57, D-37075 Göttingen, Germany.); Hufert FT ( Department of Virology, University Medical Center Göttingen, Kreuzbergring 57, D-37075 Göttingen, Germany.); Weidmann M ( Institute of Aquaculture, University of Stirling, Stirling FK9 4LA, UK.); Cardoso JF ( Center for Technological Innovation of Evandro Chagas Institute, Ministry of Health, BR 316, Km 07, s/n CEP 67.030-000, Ananindeua, Brazil.); Carvalho VL ( Departamento de Arbovirologia e Febres Hemorrágicas, Instituto Evandro Chagas, Ministério da Saúde, BR 316, Km 07, s/n CEP 67.030-000, Ananindeua, Brazil.); Chiang JO ( Departamento de Arbovirologia e Febres Hemorrágicas, Instituto Evandro Chagas, Ministério da Saúde, BR 316, Km 07, s/n CEP 67.030-000, Ananindeua, Brazil.); Martins LC ( Departamento de Arbovirologia e Febres Hemorrágicas, Instituto Evandro Chagas, Ministério da Saúde, BR 316, Km 07, s/n CEP 67.030-000, Ananindeua, Brazil.); Lima CP ( Center for Technological Innovation of Evandro Chagas Institute, Ministry of Health, BR 316, Km 07, s/n CEP 67.030-000, Ananindeua, Brazil.); Da Silva DE ( Center for Technological Innovation of Evandro Chagas Institute, Ministry of Health, BR 316, Km 07, s/n CEP 67.030-000, Ananindeua, Brazil.); Vianez-Júnior JL ( Center for Technological Innovation of Evandro Chagas Institute, Ministry of Health, BR 316, Km 07, s/n CEP 67.030-000, Ananindeua, Brazil.); Popov VL ( Department of Pathology, Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, TX 77555, USA.); Travassos da Rosa AP ( Department of Pathology, Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, TX 77555, USA.); Tesh RB ( Department of Pathology, Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, TX 77555, USA.); Vasconcelos PF ( Departamento de Arbovirologia e Febres Hemorrágicas, Instituto Evandro Chagas, Ministério da Saúde, BR 316, Km 07, s/n CEP 67.030-000, Ananindeua, Brazil Departamento de Patologia, Universidade do Estado do Pará, Belém, Brazil.); Nunes MR ( Center for Technological Innovation of Evandro Chagas Institute, Ministry of Health, BR 316, Km 07, s/n CEP 67.030-000, Ananindeua, Brazil spatroca@gmail.com marcionunes@iec.pa.gov.br.) |
| Abstract | The genus Orbivirus of the family Reoviridae comprises 22 virus species including the Changuinola virus (CGLV) serogroup. The complete genome sequences of 13 CGLV serotypes isolated between 1961 and 1988 from distinct geographical areas of the Brazilian Amazon region were obtained. All viral sequences were obtained from single-passaged CGLV strains grown in Vero cells. CGLVs are the only orbiviruses known to be transmitted by phlebotomine sandflies. Ultrastructure and molecular analysis by electron microscopy and gel electrophoresis, respectively, revealed viral particles with typical orbivirus size and morphology, as well as the presence of a segmented genome with 10 segments. Full-length nucleotide sequencing of each of the ten RNA segments of the 13 CGLV serotypes provided basic information regarding the genome organization, encoded proteins and genetic traits. Segment 2 (encoding VP2) of the CGLV is uncommonly larger in comparison to those found in other orbiviruses and shows varying sizes even among different CGLV serotypes. Phylogenetic analysis support previous serological findings, which indicate that CGLV constitutes a separate serogroup within the genus Orbivirus. In addition, six out of 13 analysed CGLV serotypes showed reassortment of their genome segments. |
| File Format | HTM / HTML |
| ISSN | 00221317 |
| Issue Number | Pt 10 |
| Volume Number | 95 |
| e-ISSN | 14652099 |
| Journal | Journal of General Virology |
| Language | English |
| Publisher | Microbiology Society |
| Publisher Date | 2014-10-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | Subscribed |
| Subject Keyword | Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't Humans Molecular Sequence Data Rna, Viral Phylogeny Journal Article Viral Structural Proteins Virion Ultrastructure Orbivirus Brazil Genetics Electrophoresis Physiology Gene Order Genome, Viral Discipline Virology Microscopy, Electron Sequence Analysis, Dna Chemistry Animals Insects Analysis Cluster Analysis |
| Content Type | Text |
| Resource Type | Article |
| Subject | Virology |
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