NDLI: Expression of viral polymerase and phosphorylation of core protein determine core and capsid localization of the human hepatitis B virus.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Deroubaix, Aurélie Osseman, Quentin Cassany, Aurélia Bégu, Dominique Ragues, Jessica Kassab, Somar Lainé, Sébastien Kann, Michael
Description	Author Affiliation: Deroubaix A ( Univ. de Bordeaux, Microbiologie Fondamentale et Pathogénicité, UMR 5234, Bordeaux, France CNRS, Microbiologie Fondamentale et Pathogénicité, UMR 5234, Bordeaux, France Hepatitis Virus Diversity Research Programme, University of the Witwatersrand, 7 York Road, Parktown, Johannesburg 2193, South Afri); Osseman Q ( Univ. de Bordeaux, Microbiologie Fondamentale et Pathogénicité, UMR 5234, Bordeaux, France CNRS, Microbiologie Fondamentale et Pathogénicité, UMR 5234, Bordeaux, France.); Cassany A ( Univ. de Bordeaux, Microbiologie Fondamentale et Pathogénicité, UMR 5234, Bordeaux, France CNRS, Microbiologie Fondamentale et Pathogénicité, UMR 5234, Bordeaux, France.); Bégu D ( Univ. de Bordeaux, Microbiologie Fondamentale et Pathogénicité, UMR 5234, Bordeaux, France CNRS, Microbiologie Fondamentale et Pathogénicité, UMR 5234, Bordeaux, France.); Ragues J ( Univ. de Bordeaux, Microbiologie Fondamentale et Pathogénicité, UMR 5234, Bordeaux, France CNRS, Microbiologie Fondamentale et Pathogénicité, UMR 5234, Bordeaux, France.); Kassab S ( Univ. de Bordeaux, Microbiologie Fondamentale et Pathogénicité, UMR 5234, Bordeaux, France CNRS, Microbiologie Fondamentale et Pathogénicité, UMR 5234, Bordeaux, France.); Lainé S ( Univ. de Bordeaux, Microbiologie Fondamentale et Pathogénicité, UMR 5234, Bordeaux, France CNRS, Microbiologie Fondamentale et Pathogénicité, UMR 5234, Bordeaux, France Université Montpellier 1, CPBS, UMR 5236 CNRS, Montpellier, France sebastien.laine@cpbs.cnrs.f.); Kann M ( Univ. de Bordeaux, Microbiologie Fondamentale et Pathogénicité, UMR 5234, Bordeaux, France CNRS, Microbiologie Fondamentale et Pathogénicité, UMR 5234, Bordeaux, France CHU de Bordeaux, Bordeaux, France.)
Abstract	Biopsies from patients show that hepadnaviral core proteins and capsids - collectively called core - are found in the nucleus and cytoplasm of infected hepatocytes. In the majority of studies, cytoplasmic core localization is related to low viraemia while nuclear core localization is associated with high viral loads. In order to better understand the molecular interactions leading to core localization, we analysed transfected hepatoma cells using immune fluorescence microscopy. We observed that expression of core protein in the absence of other viral proteins led to nuclear localization of core protein and capsids, while expression of core in the context of the other viral proteins resulted in a predominantly cytoplasmic localization. Analysis of which viral partner was responsible for cytoplasmic retention indicated that the HBx, surface proteins and HBeAg had no impact but that the viral polymerase was the major determinant. Further analysis revealed that Ïµ, an RNA structure to which the viral polymerase binds, was essential for cytoplasmic retention. Furthermore, we showed that core protein phosphorylation at Ser 164 was essential for the cytoplasmic core localization phenotype, which is likely to explain differences observed between individual cells.
File Format	HTM / HTML
ISSN	00221317
Issue Number	Pt 1
Volume Number	96
e-ISSN	14652099
Journal	Journal of General Virology
Language	English
Publisher	Microbiology Society
Publisher Date	2015-01-01
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Research Support, Non-u.s. Gov't Phosphorylation Hepatitis B e Antigens Hepatitis B Virus Discipline Virology Humans Metabolism Viral Core Proteins Journal Article Hep G2 Cells Transfection Capsid Proteins Cell Line, Tumor Genetics Dna-directed Dna Polymerase Cell Nucleus Cytoplasm Methods
Content Type	Text
Resource Type	Article
Subject	Virology

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