NDLI: Genetic variation and dynamics of infections of equid herpesvirus 5 in individual horses.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Back, Helena Ullman, Karin Leijon, Mikael Söderlund, Robert Penell, Johanna Ståhl, Karl Pringle, John Valarcher, Jean-François
Description	Country affiliation: Sweden Author Affiliation: Back H ( 1âDepartment of Virology, Immunobiology and Parasitology, National Veterinary Institute, Uppsala, Sweden.); Ullman K ( 1âDepartment of Virology, Immunobiology and Parasitology, National Veterinary Institute, Uppsala, Sweden.); Leijon M ( 1âDepartment of Virology, Immunobiology and Parasitology, National Veterinary Institute, Uppsala, Sweden.); Söderlund R ( 2âDepartment of Bacteriology, National Veterinary Institute, Uppsala, Sweden.); Penell J ( 3âDepartment of Veterinary Epidemiology and Public Health, University of Surrey, Guildford, UK.); Ståhl K ( 4âDepartment of Disease Control and Epidemiology, National Veterinary Institute, Uppsala, Sweden.); Pringle J ( 5âDepartment of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.); Valarcher JF ( 1âDepartment of Virology, Immunobiology and Parasitology, National Veterinary Institute, Uppsala, Sweden 5âDepartment of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.)
Abstract	Equid herpesvirus 5 (EHV-5) is related to the human Epstein-Barr virus (human herpesvirus 4) and has frequently been observed in equine populations worldwide. EHV-5 was previously assumed to be low to non-pathogenic; however, studies have also related the virus to the severe lung disease equine multinodular pulmonary fibrosis (EMPF). Genetic information of EHV-5 is scanty: the whole genome was recently described and only limited nucleotide sequences are available. In this study, samples were taken twice 1 year apart from eight healthy horses at the same professional training yard and samples from a ninth horse that was diagnosed with EMPF with samples taken pre- and post-mortem to analyse partial glycoprotein B (gB) gene of EHV-5 by using next-generation sequencing. The analysis resulted in 27 partial gB gene sequences, 11 unique sequence types and five amino acid sequences. These sequences could be classified within four genotypes (I-IV) of the EHV-5 gB gene based on the degree of similarity of the nucleotide and amino acid sequences, and in this work horses were shown to be identified with up to three different genotypes simultaneously. The observations showed a range of interactions between EHV-5 and the host over time, where the same virus persists in some horses, whereas others have a more dynamic infection pattern including strains from different genotypes. This study provides insight into the genetic variation and dynamics of EHV-5, and highlights that further work is needed to understand the EHV-5 interaction with its host.
File Format	HTM / HTML
ISSN	00221317
Issue Number	1
Volume Number	97
e-ISSN	14652099
Journal	Journal of General Virology
Language	English
Publisher	Microbiology Society
Publisher Date	2016-01-01
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Research Support, Non-u.s. Gov't Coinfection Discipline Virology Herpesviridae Isolation & Purification Molecular Sequence Data Dna, Viral Genotype Phylogeny Sequence Analysis, Dna Journal Article Virology Genetic Variation Sequence Homology Herpesviridae Infections Horse Diseases Chemistry Animals Classification Horses Genetics Veterinary Carrier State Cluster Analysis
Content Type	Text
Resource Type	Article
Subject	Virology

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