NDLI: Pro-inflammatory cytokine dysregulation is associated with novel avian influenza A (H7N9) virus in primary human macrophages.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Zhao, Chihao Qi, Xian Ding, Meng Sun, Xinlei Zhou, Zhen Zhang, Shuo Zen, Ke Li, Xihan
Description	Author Affiliation: Zhao C ( 1â State Key Laboratory of Pharmaceutical Biotechnology, Nanjing Advanced Institute for Life Sciences (NAILS), Nanjing University, 22 Hankou Road, Nanjing, Jiangsu 210093, PR China.); Qi X ( 2â Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, Jiangsu 210009, PR China.); Ding M ( 1â State Key Laboratory of Pharmaceutical Biotechnology, Nanjing Advanced Institute for Life Sciences (NAILS), Nanjing University, 22 Hankou Road, Nanjing, Jiangsu 210093, PR China.); Sun X ( 1â State Key Laboratory of Pharmaceutical Biotechnology, Nanjing Advanced Institute for Life Sciences (NAILS), Nanjing University, 22 Hankou Road, Nanjing, Jiangsu 210093, PR China.); Zhou Z ( 1â State Key Laboratory of Pharmaceutical Biotechnology, Nanjing Advanced Institute for Life Sciences (NAILS), Nanjing University, 22 Hankou Road, Nanjing, Jiangsu 210093, PR China.); Zhang S ( 1â State Key Laboratory of Pharmaceutical Biotechnology, Nanjing Advanced Institute for Life Sciences (NAILS), Nanjing University, 22 Hankou Road, Nanjing, Jiangsu 210093, PR China.); Zen K ( 1â State Key Laboratory of Pharmaceutical Biotechnology, Nanjing Advanced Institute for Life Sciences (NAILS), Nanjing University, 22 Hankou Road, Nanjing, Jiangsu 210093, PR China.); Li X ( 1â State Key Laboratory of Pharmaceutical Biotechnology, Nanjing Advanced Institute for Life Sciences (NAILS), Nanjing University, 22 Hankou Road, Nanjing, Jiangsu 210093, PR China.)
Abstract	Since March 2013, more than 500 laboratory-confirmed human H7N9 influenza A virus infection cases have been recorded, with a case fatality rate of more than 30%. Clinical research has shown that cytokine and chemokine dysregulation contributes to the pathogenicity of the H7N9 virus. Here, we investigated cytokine profiles in primary human macrophages infected with the novel H7N9 virus, using cytokine antibody arrays. The levels of several pro-inflammatory cytokines, particularly TNF- , were increased in H7N9-infected macrophages. Induction of the transcriptional and translational levels of the pro-inflammatory cytokines by H7N9 virus seemed to be intermediate between those induced by highly pathogenic avian H5N1 and pandemic human H1N1 viruses, which were detected by ELISA and real-time quantitative PCR, respectively. Additionally, compared with H5N1, the upregulation of pro-inflammatory cytokines caused by H7N9 infection occurred rapidly but mildly. Our results identified the overall profiles of cytokine and chemokine induction by the H7N9 influenza virus in an in vitro cell-culture model, and could provide potential therapeutic targets for the control of severe human H7N9 disease.
File Format	HTM / HTML
ISSN	00221317
Issue Number	2
Volume Number	97
e-ISSN	14652099
Journal	Journal of General Virology
Language	English
Publisher	Microbiology Society
Publisher Date	2016-02-01
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Research Support, Non-u.s. Gov't Cytokines Discipline Virology Humans Cells, Cultured Isolation & Purification Real-time Polymerase Chain Reaction Birds Gene Expression Profiling Influenza A Virus, H1n1 Subtype Macrophages Enzyme-linked Immunosorbent Assay Journal Article Virology Growth & Development Immunology Animals Analysis Influenza A Virus, H7n9 Subtype Protein Array Analysis Influenza A Virus, H5n1 Subtype Influenza In Birds
Content Type	Text
Resource Type	Article
Subject	Virology

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