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Year: 2010 Volume: 55 Issue: 2

Year: 2010 Volume: 55 Issue: 1

Year: 2009 Volume: 54

Year: 2008 Volume: 53

Age estimation by racemization method in teeth: application of aspartic acid, glutamate, and alanine.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Arany, Szilvia Ohtani, Susumu
Description	Country affiliation: Japan Author Affiliation: Arany S ( Department of Biochemistry, Akita University School of Medicine, Akita, Japan. aszilvia@med.akita-u.ac.jp)
Abstract	Here, we report on an experimental approach of simultaneous determination of various amino acids racemization (AAR) rates in teeth. We evaluated the measurements of aspartic acid (Asp), glutamate (Glu), and alanine (Ala) isolated from dentin. Asx D/L rates from total amino acid fraction, generally used for age estimation, showed high correlation (r = 0.98) with age. As Glx and Ala showed very slow racemization kinetics in TA, we performed further analysis of the acid-soluble protein (SP) fraction. The results supported improved correlation between age and D/L rates for Glu (r = 0.84) and Ala (r = 0.85), as well as for Asp (r = 0.98). By providing further elucidation on dentin protein racemization, the technique offers a considerable opportunity to involve other amino acids in age estimation studies. As the process does not require additional separation steps, the method can be easily adapted to existing protocols.
File Format	HTM / HTML
ISSN	00221198
Issue Number	3
Volume Number	55
e-ISSN	15564029
Journal	Journal of Forensic Sciences
Language	English
Publisher	Wiley-Blackwell
Publisher Date	2010-05-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Dentin Alanine Bicuspid Biological Markers Humans Middle Aged Age Determination By Teeth Linear Models Discipline Forensic Sciences Aspartic Acid Aged, 80 And Over Journal Article Glutamic Acid Young Adult Chemistry Forensic Dentistry Flame Ionization Analysis Adolescent Adult Aged Methods
Content Type	Text
Resource Type	Article
Subject	Genetics Pathology and Forensic Medicine

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