NDLI: In vitro evaluation of the bioactive factors preserved in porcine small intestinal submucosa through cellular biological approaches.

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In vitro evaluation of the bioactive factors preserved in porcine small intestinal submucosa through cellular biological approaches.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Yang, Bin Zhou, Liuhua Sun, Zeyu Yang, Rong Chen, Yun Dai, Yutian
Description	Author Affiliation: Yang B ( Department of Urology, Affiliated Drum Tower Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu 210008, People's Republic of China.)
Abstract	The objective of this study was to develop cellular biological approaches to evaluate the potential effect of bioactive factors in porcine small intestinal submucosa (SIS) on bladder regeneration and angiogenesis. For this purpose, we cultured human bladder smooth muscle cell (HBSMC) and human umbilical vein endothelial cell (HUVEC), and then used cellular biological techniques to characterize in vitro biological effect of SIS components on HBSMC and HUVEC. Our results indicated that the SIS components had stimulated the attachment, proliferation, and migration of HBSMC and HUVEC, as well as tube formation by HUVEC on Matrigel. These results implied that the SIS might have preserved a mixture of bioactive factors including cell adhesion factors, mitogenic factors, chemotactic cytokines, and angiogenic factors, and these bioactive factors would have the potential of promoting bladder regeneration and angiogenesis. In conclusion, these cellular biological approaches might be helpful and effective for evaluation of the bioactive factors preserved in porcine SIS before it is used for bladder augmentation in humans.
File Format	HTM / HTML
ISSN	15493296
Issue Number	3
Volume Number	93
e-ISSN	15524965
Journal	Journal of Biomedical Materials Research Part A
Language	English
Publisher	Wiley
Publisher Date	2010-06-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biological Factors Intestinal Mucosa Cell Proliferation Endothelial Cells Intestine, Small Humans Cells, Cultured Cytology Umbilical Veins Pharmacology Metabolism Cell Adhesion Drug Effects Journal Article Discipline Biomedical Engineering Culture Media, Conditioned Animals Myocytes, Smooth Muscle Sus Scrofa Urinary Bladder Methods Neovascularization, Physiologic Tissue Engineering Cell Movement
Content Type	Text
Resource Type	Article
Subject	Ceramics and Composites Metals and Alloys Biomaterials Biomedical Engineering

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