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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Karadzic, Ivana Vucic, Vesna Jokanovic, Vukoman Debeljak-Martacic, Jasmina Markovic, Dejan Petrovic, Snjezana Glibetic, Marija |
| Description | Author Affiliation: Karadzic I ( Centre of Research Excellence in Nutrition and Metabolism, Institute for Medical Research, University of Belgrade, Belgrade, 11000, Serbia.) |
| Abstract | The aim of this study was to examine the differential capacity of isolated dental pulp stem cells (SHED) cultured onto four different scaffold materials. The differential potential of isolated SHED was examined on the following scaffolds: porous hydroxyapatite (pHAP) alone or combined with three polymers [polylactic-co-glycolic acid (PLGA), alginate, and ethylene vinylacetate / ethylene vinylversatate (EVA/EVV)]. SHED were isolated by 'outgrowth' method and characterized by the flow cytometry. Viability of cells grown with scaffolds was assessed by MTT and LDH assays. No significant cytotoxic effect of any of the tested materials was shown. Staining with alizarin red and estimated alkaline phosphatase activity to identify differentiation, demonstrated osteoblastic phenotype of SHED and newly deposited and mineralized extra cellular matrix (ECM) in presence of all tested scaffolds. The developed ECM seen at scanning electronic micrographs additionally confirmed the osteogenic differentiation and biocompatibility between cells and materials. In summary, all studied biomaterials are suitable carriers for proliferation and osteoblastic differentiation of dental pulp mesenchymal stem cells in vitro. |
| File Format | HTM / HTML |
| ISSN | 15493296 |
| Issue Number | 1 |
| Volume Number | 103 |
| e-ISSN | 15524965 |
| Journal | Journal of Biomedical Materials Research Part A |
| Language | English |
| Publisher | Wiley |
| Publisher Date | 2015-01-01 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Durapatite Research Support, Non-u.s. Gov't Microscopy, Electron, Scanning Cell Proliferation Humans Cytology Osteoblasts Journal Article Discipline Biomedical Engineering Chemistry Flow Cytometry Mesenchymal Stromal Cells Biocompatible Materials Cell Differentiation Polymers Child |
| Content Type | Text |
| Resource Type | Article |
| Subject | Ceramics and Composites Metals and Alloys Biomaterials Biomedical Engineering |
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