NDLI: Electrospun gelatin biopapers as substrate for in vitro bilayer models of blood-brain barrier tissue.

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Electrospun gelatin biopapers as substrate for in vitro bilayer models of blood-brain barrier tissue.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Bischel, Lauren L. Coneski, Peter N. Lundin, Jeffrey G. Wu, Peter K. Giller, Carl B. Wynne, James Ringeisen, Brad R. Pirlo, Russell K.
Description	Author Affiliation: Bischel LL ( American Society for Engineering Education Postdoctoral Fellow at the U.S. Naval Research Laboratory, Washington, DC.); Coneski PN ( American Society for Engineering Education Postdoctoral Fellow at the U.S. Naval Research Laboratory, Washington, DC.); Lundin JG ( U.S. Naval Research Laboratory, Chemistry Division, Washington, DC.); Wu PK ( Department of Physics, Southern Oregon University, Ashland, Oregon.); Giller CB ( Contractor at the U.S. Naval Research Laboratory, Leidos, Washington, DC.); Wynne J ( U.S. Naval Research Laboratory, Chemistry Division, Washington, DC.); Ringeisen BR ( U.S. Naval Research Laboratory, Chemistry Division, Washington, DC.); Pirlo RK ( U.S. Naval Research Laboratory, Chemistry Division, Washington, DC.)
Abstract	Gaining a greater understanding of the blood-brain barrier (BBB) is critical for improvement in drug delivery, understanding pathologies that compromise the BBB, and developing therapies to protect the BBB. In vitro human tissue models are valuable tools for studying these issues. The standard in vitro BBB models use commercially available cell culture inserts to generate bilayer co-cultures of astrocytes and endothelial cells (EC). Electrospinning can be used to produce customized cell culture substrates with optimized material composition and mechanical properties with advantages over off-the-shelf materials. Electrospun gelatin is an ideal cell culture substrate because it is a natural polymer that can aid cell attachment and be modified and degraded by cells. Here, we have developed a method to produce cell culture inserts with electrospun gelatin 'biopaper' membranes. The electrospun fiber diameter and cross-linking method were optimized for the growth of primary human endothelial cell and primary human astrocyte bilayer co-cultures to model human BBB tissue. BBB co-cultures on biopaper were characterized via cell morphology, trans-endothelial electrical resistance (TEER), and permeability to FITC-labeled dextran and compared to BBB co-cultures on standard cell culture inserts. Over longer culture periods (up to 21 days), cultures on the optimized electrospun gelatin biopapers were found to have improved TEER, decreased permeability, and permitted a smaller separation between co-cultured cells when compared to standard PET inserts.
File Format	HTM / HTML
ISSN	15493296
Issue Number	4
Journal	Journal of Biomedical Materials Research Part A
Volume Number	104
e-ISSN	15524965
Language	English
Publisher	Wiley
Publisher Date	2016-04-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Biomedical Engineering
Content Type	Text
Resource Type	Article
Subject	Ceramics and Composites Metals and Alloys Biomaterials Biomedical Engineering

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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