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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Akineden, Omer Hassan, Abdulwahed Ahmed Schneider, Elisabeth Usleber, Ewald |
| Description | Country affiliation: Germany Author Affiliation: Akineden O ( Dairy Sciences, Institute of Veterinary Food Sciences, Justus-Liebig-University Giessen, Ludwigstrasse 21, Giessen, Germany. Oemer.Akineden@vetmed.uni-giessen.de) |
| Abstract | Goats' milk cheeses (n=181) from the Hessian market (retail shops, weekly markets, farm markets) were quantitatively analysed for Staphylococcus (S.) aureus, and 14 were found positive. From these samples, 64 isolates of S. aureus were characterized biochemically and genetically, including their potential to produce staphylococcal enterotoxins (SE). SE genes sea to selo was studied by PCR and gene expression was evaluated by reverse transcriptase (RT)-PCR. SEA-SEE production in culture was determined by enzyme immunoassay (EIA). One isolate produced SEA, 18 isolates (from 4 samples) produced SEC, while SEB, SED, and SEE were not found. Toxin production was in agreement with PCR and RT-PCR results for the presence and expression, respectively, of the corresponding toxin genes. Trans-SE genes seg, sei, selm, seln, and selo were detected in 14 isolates from 4 cheese samples, exclusively as clusters. These samples were all from small-scale producers which directly or indirectly market their products regionally. No isolate was positive for seh or sej. RT-PCR detected the presence of the corresponding mRNA for all genes except selo, further indicating the possibility that respective proteins indeed have been produced in culture. These results suggest that S. aureus in goats' milk cheese potentially produces SE like proteins, besides SEA and SEC. |
| File Format | HTM / HTML |
| ISSN | 01681605 |
| Issue Number | 2 |
| Volume Number | 124 |
| e-ISSN | 18793460 |
| Journal | International Journal of Food Microbiology |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2008-05-31 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Microbiology Microbiology Goats Enterotoxins Food Contamination Consumer Product Safety Humans Isolation & Purification Cheese Biosynthesis Colony Count, Microbial Reverse Transcriptase Polymerase Chain Reaction Metabolism Food Microbiology Journal Article Chemistry Animals Dna, Bacterial Analysis Polymerase Chain Reaction Genetics Staphylococcus Aureus Discipline Nutritional Sciences Milk |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Microbiology Safety, Risk, Reliability and Quality Food Science |
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