NDLI: Isolation and molecular identification of Cronobacter spp. from powdered infant formula (PIF) in Bangladesh.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Hoque, Ashfaqul Ahmed, Tahmeed Shahidullah, Mohammad Hossain, Anowar Mannan, Abdul Noor, Khaled Nahar, Kamrun Ilias, Mohammad Ahmed, Dilruba
Spatial Coverage	Bangladesh
Description	Country affiliation: Bangladesh Author Affiliation: Hoque A ( International Center for Diarrhoeal Disease Research, Bangladesh (ICDDR,B), Dhaka, Bangladesh.)
Abstract	Cronobacter spp. formerly known as Enterobacter sakazakii is an occasional contaminant of powdered infant formula (PIF). This pathogen has been associated with out-breaks of a rare form of infant meningitis, necrotizing enterocolitis (NEC), bacteremia and neonate deaths. The organism is ranked by the International Commission for Microbiological Specifications for Foods (ICMSF) as a 'Severe hazard for restricted populations, life threatening or substantial chronic sequelae or long duration'. Present study aimed to isolate Cronobacter spp. from PIF and clinical samples, such as blood, stool and CSF collected from 93 neonates and child patients, age ranged from 0 to 24months. We did not detect Cronobacter spp. in any of these samples. Later 32 PIF samples collected from retail markets in Bangladesh were tested for the presence of Cronobacter spp. Of these only one was found to be contaminated with Cronobacter sp. This is the first case of Cronobacter contaminated PIF found in Bangladesh to be reported. The organism was successfully identified based on its typical culture characteristics, producing blue-green colonies on chromogenic DFI agar and also by a standardized conventional PCR assay targeting the alpha glucosidase and 16S rRNA gene sequence of Cronobacter sp. The 16S rRNA gene was partially sequenced to provide for the phylogenetic analysis of this isolate (DA01) and found to cluster with some other Cronobacter isolates in the phylogram.
File Format	HTM / HTML
ISSN	01681605
Issue Number	3
Volume Number	142
e-ISSN	18793460
Journal	International Journal of Food Microbiology
Language	English
Publisher	Elsevier
Publisher Date	2010-09-01
Publisher Place	Netherlands
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Research Support, Non-u.s. Gov't Microbiology Rna, Bacterial Rna, Ribosomal, 16s Food Contamination Humans Infant Food Molecular Sequence Data Infant Phylogeny Cronobacter Sakazakii Food Microbiology Journal Article Base Sequence Classification Polymerase Chain Reaction Genetics Feces Infant, Newborn Discipline Microbiology Infant Formula Consumer Product Safety Isolation & Purification Bangladesh Analysis Discipline Nutritional Sciences Cluster Analysis
Content Type	Text
Resource Type	Article
Subject	Medicine Microbiology Safety, Risk, Reliability and Quality Food Science

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Actual distribution of Cronobacter spp. in industrial batches of powdered infant formula and consequences for performance of sampling strategies.

Development of an immobilization and detection method of Enterobacter sakazakii from powdered infant formula.

Prevalence of Cronobacter spp. in a powdered infant formula processing environment.

Growth and survival of Enterobacter sakazakii in human breast milk with and without fortifiers as compared to powdered infant formula.

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Genotypic and phenotypic characterisation of a collection of Cronobacter (Enterobacter sakazakii) isolates.

Biochemical and molecular characterization of Cronobacter spp. (formerly Enterobacter sakazakii) isolated from foods.

Enterobacter sakazakii (Cronobacter spp.) in powdered follow-up formulae : meeting report

A New Validated Real-Time PCR-Based Method for the Specific and Fast Detection of Cronobacter spp. in Infant Formula

Isolation and molecular identification of Cronobacter spp. from powdered infant formula (PIF) in Bangladesh.

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Actual distribution of Cronobacter spp. in industrial batches of powdered infant formula and consequences for performance of sampling strategies.

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Growth and survival of Enterobacter sakazakii in human breast milk with and without fortifiers as compared to powdered infant formula.

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