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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Chang, Yuhua Gu, Weimin Zhang, Fujia McLandsborough, Lynne |
| Description | Country affiliation: United States Author Affiliation: Chang Y ( Department of Food Science, University of Massachusetts, Amherst, MA 01003, USA.) |
| Abstract | The distribution and survival of Listeria monocytogenes (L. monocytogenes) in food processing environment is linked to its ability to form biofilms, however the genetic mechanisms remain unclear. In our previous study, a Himar1 mariner-based transposon mutagenesis was performed and 42 mutants were confirmed to have reduced biofilm formation. Among the 42 biofilm deficient mutants, two isolates (s25-10C and s55-1D) harbored single insertion in lmo1386, a gene encoding a putative DNA translocase. The lmo1386 mutants had impaired biofilm formation in both static and flow conditions. The mutant strain s55-1D was complemented by cloning the entire lmo1386 gene into pPL2-gtcAP, a derivative of the integration vector pPL2 with the L. monocytogenes gtcA promoter. The genetically complemented mutant restored its biofilm phenotype, demonstrating the role of lmo1386 in the biofilm formation of L. monocytogenes. The lmo1386 mutant had reduced initial adhesion ability, which could at least partially contribute to the impaired biofilm phenotype. Additionally, the lmo1386 mutant formed elongated cell chains when grown in a nutrient TSBYE media, while no obvious cell morphology changes were observed when grown in the minimal MWB media. Overall, our findings suggest that the disruption of lmo1386, a putative DNA translocase gene affects the biofilm formation of L. monocytogenes on abiotic surfaces, which may further advance the understanding of the complicated process of biofilm formation. |
| File Format | HTM / HTML |
| ISSN | 01681605 |
| Issue Number | 3 |
| Volume Number | 161 |
| e-ISSN | 18793460 |
| Journal | International Journal of Food Microbiology |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2013-02-15 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Research Support, U.s. Gov't, Non-p.h.s. Research Support, Non-u.s. Gov't Discipline Microbiology Listeria Monocytogenes Nucleobase, Nucleoside, Nucleotide, And Nucleic Acid Transport Proteins Metabolism Journal Article Bacterial Proteins Bacterial Adhesion Growth & Development Biofilms Dna, Bacterial Cloning, Molecular Genetics Mutagenesis, Insertional Discipline Nutritional Sciences |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Microbiology Safety, Risk, Reliability and Quality Food Science |
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