NDLI: Metal distribution and metallothionein induction after cadmium exposure in the terrestrial snail Helix aspersa (Gastropoda, $Pulmonata)^{†}$

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Metal distribution and metallothionein induction after cadmium exposure in the terrestrial snail Helix aspersa (Gastropoda, $Pulmonata)^{†}$

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Metal distribution and metallothionein induction after cadmium exposure in the terrestrial snail Helix aspersa (Gastropoda, $Pulmonata)^{†}$

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Hispard, Florian Schuler, Dietmar de Vaufleury, Annette Scheifler, Renaud Badot, Pierre-Marie Dallinger, Reinhard
Description	Country affiliation: France Author Affiliation: Hispard F ( University of Franche-Comté, Department of Chrono-Environment, Mixed Research Unit 6249, University of Franche-Comté, Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique, Place Leclerc, 25030 Besançon Cedex, France.)
Abstract	The aim of the present work was to study the effect of $Cd^{2+}$ exposure on metallothionein (MT) induction and on the distribution of metals (Cd, Cu, and Zn) in the terrestrial pulmonate Helix aspersa. In particular, the soluble and nonsoluble pools of the accumulated metals and their tissue distribution in uncontaminated and contaminated edible snails were investigated after a two-week exposure to $Cd^{2+}.$ In the soluble cytosolic pool of the midgut gland of H. aspersa, three metal-specific putative MT isoforms were separated following a fractionation protocol with diethylaminoethyl cellulose, size-exclusion chromatography, ultrafiltration, and reversed-phase high-performance liquid chromatography (RP-HPLC). Interestingly, one of the above isoforms seems to bind both Cd and Cu, which may in addition mobilize, after induction by $Cd^{2+},$ some of the intracellular Cu and, thus, perhaps increase the Cu pool in the cytosolic fraction. The cDNA and its translated amino acid sequence of a $Cd^{2+}-binding$ MT isoform from the snail midgut gland was characterized and attributed to one of the putative MT isoforms obtained by RP-HPLC. The amino acid sequence of this Cd-MT isoform of H. aspersa differed from similar sequences described in other terrestrial pulmonates, such as Helix pomatia or Arianta arbustorum, by only a few amino acids (n = 4 and 8, respectively). That the identified Cd-MT from H. aspersa is inducible by $Cd^{2+}$ also was shown, chromatographic evidence aside, by a specific polymerase chain reaction protocol on a cDNA basis, which included a noninducible housekeeping gene as a control.
File Format	HTM / HTML
ISSN	07307268
Issue Number	7
Volume Number	27
e-ISSN	15528618
Journal	Environmental Toxicology and Chemistry
Language	English
Publisher	Wiley
Publisher Date	2008-07-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Research Support, Non-u.s. Gov't Discipline Environmental Health Dna, Complementary Molecular Sequence Data Discipline Toxicology Metals, Heavy Chromatography, High Pressure Liquid Deae-cellulose Journal Article Tissue Distribution Ultrafiltration Time Factors Electrophoresis, Agar Gel Base Sequence Polymerase Chain Reaction Discipline Chemistry Genetics Helix (snails) Copper Cadmium Amino Acid Sequence Salivary Glands Solubility Metabolism Drug Effects Zinc Chemistry Animals Analysis Protein Isoforms Pharmacokinetics Metallothionein Methods
Content Type	Text
Resource Type	Article
Subject	Environmental Chemistry Health, Toxicology and Mutagenesis

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