NDLI: Characterization of acetylcholinesterase from the brain of the Amazonian tambaqui (Colossoma macropomum) and in vitro effect of organophosphorus and carbamate pesticides.

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Characterization of acetylcholinesterase from the brain of the Amazonian tambaqui (Colossoma macropomum) and in vitro effect of organophosphorus and carbamate pesticides.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Assis, Caio Rodrigo Dias Castro, Patrícia Fernandes Amaral, Ian Porto Gurgel Carvalho, Elba Verônica Matoso Maciel Carvalho, Luiz Bezerra Bezerra, Ranilson Souza
Description	Country affiliation: Brazil Author Affiliation: Assis CR ( Laboratório de Enzimologia, LABENZ, Departamento de Bioquímica and Laboratório de Imunopatologia Keizo Asami, Universidade Federal de Pernambuco, Pernambuco, Brazil.)
Abstract	In the present study, acetylcholinesterase (AChE) from the brain of the Amazonian fish tambaqui (Colossoma macropomum) was partially characterized and its activity was assayed in the presence of five organophosphates (dichlorvos, diazinon, chlorpyrifos, and tetraethyl pyrophosphate [TEPP]) and two carbamates (carbaryl and carbofuran) insecticides. Optimal pH and temperature were 7.0 to 8.0 and 45°C, respectively. The enzyme retained approximately 70% of activity after incubation at 50°C for 30 min. The insecticide concentration capable of inhibiting half of the enzyme activity (IC50) for dichlorvos, chlorpyrifos, and TEPP were calculated as 0.04 µmol/L, 7.6 µmol/L, and 3.7 µmol/L, respectively. Diazinon and temephos did not inhibit the enzyme. The IC50 values for carbaryl and carbofuran were estimated as 33.8 µmol/L and 0.92 µmol/L, respectively. These results suggest that AChE from the juvenile C. macropomum brain could be used as an alternative biocomponent of organophosphorus and carbamate biosensors in routine pesticide screening in the environment.
File Format	HTM / HTML
ISSN	07307268
Issue Number	10
Volume Number	29
e-ISSN	15528618
Journal	Environmental Toxicology and Chemistry
Language	English
Publisher	Wiley
Publisher Date	2010-10-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Brain Research Support, Non-u.s. Gov't Temperature Cholinesterase Inhibitors Species Specificity Toxicity Discipline Environmental Health Pesticides Organophosphorus Compounds Discipline Toxicology Acetylcholinesterase Metabolism Drug Effects Journal Article Animals Fishes Biosensing Techniques Carbamates Discipline Chemistry Hydrogen-ion Concentration Enzymology In Vitro Techniques
Content Type	Text
Resource Type	Article
Subject	Environmental Chemistry Health, Toxicology and Mutagenesis

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Characterization of acetylcholinesterase from the brain of the Amazonian tambaqui (Colossoma macropomum) and in vitro effect of organophosphorus and carbamate pesticides.

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