NDLI: Quantification of adult T-cell leukemia/lymphoma cells using simple four-color flow cytometry.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Ishigaki, Tomohiro Zaike, Yuji Nojima, Masanori Kobayashi, Seiichiro Ohno, Nobuhiro Uchimaru, Kaoru Tojo, Arinobu Nakauchi, Hiromitsu Watanabe, Nobukazu
Abstract	BACKGROUND: The absolute number of adult T-cell leukemia/lymphoma (ATL) cells in peripheral blood is an essential indicator to evaluate disease status. However, microscopically counting ATL cells based on morphology requires experience and tends to be inaccurate due to the rarity of ATL. METHODS: Based on our research showing that acute-type ATL cells are specifically enriched in the CD4+/CD7- (CD7N) fraction, a new analytical method to accurately quantify ATL cells was established using an internal bead standard and simple four-color flow cytometry. This method was verified by comparison with microscopic examination of 49 peripheral blood samples and used to follow up patients. RESULTS: A strong correlation was observed between the number of CD7N cells measured by flow cytometry and the number of abnormal lymphocytes measured microscopically by experienced technicians [Pearson's R, 0.963; Spearman's rho, 0.921; intercorrelation coefficient, 0.962]. The linear regression coefficient was close to 1 (ß=1.013). Our method could detect 1 cell/µL, and the limit of quantitation was between 2.9 and 9.8 cells/µL. The frequency of CD7N cells among CD4+ cells changed during chemotherapy, which reflected differences between chemosensitive and chemoresistant cases. Kaplan-Meier analysis with a log-rank test showed that patients with decreased CD7N proportion after chemotherapy had significantly longer disease-specific survival (p=0.003). CONCLUSIONS: Our newly established method quantified tumor cells in patients with acute-type ATL. Furthermore, this method was useful for assessing the efficacy of chemotherapy, and the change of the CD7N proportion could be more important to predict prognosis.
File Format	HTM / HTML
ISSN	14346621
Issue Number	1
Volume Number	53
e-ISSN	14374331
Journal	Clinical Chemistry and Laboratory Medicine
Language	English
Publisher	Walter de Gruyter
Publisher Date	2015-01-01
Publisher Place	Germany
Access Restriction	Subscribed
Subject Keyword	Discipline Clinical chemistry Antigens, Cd4 Cell Count Humans Middle Aged Color Drug Therapy Male Treatment Outcome Metabolism Journal Article Pathology Flow Cytometry Discipline Clinical Chemistry Survival Analysis Adult Female Aged Leukemia-lymphoma, Adult T-cell Methods
Content Type	Text
Resource Type	Article
Subject	Biochemistry (medical) Clinical Biochemistry

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