NDLI: Nitric oxide is required for the auxin-induced activation of NADPH-dependent thioredoxin reductase and protein denitrosylation during root growth responses in arabidopsis.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Correa-Aragunde, Natalia Cejudo, Francisco J. Lamattina, Lorenzo
Description	Author Affiliation: Correa-Aragunde N ( Instituto de Investigaciones Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata. CC 1245, 7600 Mar del Plata, Argentina and.); Cejudo FJ ( Instituto de Bioquímica Vegetal y Fotosíntesis, Universidad de Sevilla and CSIC, Avda. Américo Vespucio 49, 41092 Sevilla, Spain.); Lamattina L ( Instituto de Investigaciones Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata. CC 1245, 7600 Mar del Plata, Argentina and lolama@mdp.edu.ar.)
Abstract	BACKGROUND AND AIMS: Auxin is the main phytohormone controlling root development in plants. This study uses pharmacological and genetic approaches to examine the role of auxin and nitric oxide (NO) in the activation of NADPH-dependent thioredoxin reductase (NTR), and the effect that this activity has on root growth responses in Arabidopsis thaliana. METHODS: Arabidopsis seedlings were treated with auxin with or without the NTR inhibitors auranofin (ANF) and 1-chloro-2, 4-dinitrobenzene (DNCB). NTR activity, lateral root (LR) formation and S-nitrosothiol content were measured in roots. Protein S-nitrosylation was analysed by the biotin switch method in wild-type arabidopsis and in the double mutant ntra ntrb. KEY RESULTS: The auxin-mediated induction of NTR activity is inhibited by the NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (CPTIO), suggesting that NO is downstream of auxin in this regulatory pathway. The NTR inhibitors ANF and DNCB prevent auxin-mediated activation of NTR and LR formation. Moreover, ANF and DNCB also inhibit auxin-induced DR5 : : GUS and BA3 : : GUS gene expression, suggesting that the auxin signalling pathway is compromised without full NTR activity. Treatment of roots with ANF and DNCB increases total nitrosothiols (SNO) content and protein S-nitrosylation, suggesting a role of the NTR-thioredoxin (Trx)-redox system in protein denitrosylation. In agreement with these results, the level of S-nitrosylated proteins is increased in the arabidopsis double mutant ntra ntrb as compared with the wild-type. CONCLUSIONS: The results support for the idea that NTR is involved in protein denitrosylation during auxin-mediated root development. The fact that a high NO concentration induces NTR activity suggests that a feedback mechanism to control massive and unregulated protein S-nitrosylation could be operating in plant cells.
File Format	HTM / HTML
ISSN	03057364
e-ISSN	10958290
DOI	10.1093/aob/mcv116
Journal	Annals of Botany
Issue Number	4
Volume Number	116
Language	English
Publisher	Oxford University Press
Publisher Date	2015-09-01
Publisher Place	Great Britain (UK)
Access Restriction	Open
Subject Keyword	Research Support, N.i.h., Extramural Discipline Botany Arabidopsis Nitric Oxide Metabolism Plant Growth Regulators Thioredoxin-disulfide Reductase Growth & Development Arabidopsis Proteins Plant Roots Genetics Indoleacetic Acids
Content Type	Text
Resource Type	Article
Subject	Plant Science

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