NDLI: $P2X_{1}$ receptor blockade inhibits whole kidney autoregulation of renal blood flow in vivo

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Osmond, David A. Inscho, Edward W.
Description	Country affiliation: United States Author Affiliation: Osmond DA ( Department of Physiology, Medical College of Georgia, Augusta, Georgia 30912, USA.)
Abstract	In vitro experiments demonstrate that P2X(1) receptor activation is important for normal afferent arteriolar autoregulatory behavior, but direct in vivo evidence for this relationship occurring in the whole kidney is unavailable. Experiments were performed to test the hypothesis that P2X(1) receptors are important for autoregulation of whole kidney blood flow. Renal blood flow (RBF) was measured in anesthetized male Sprague-Dawley rats before and during P2 receptor blockade with PPADS, P2X(1) receptor blockade with IP5I, or A(1) receptor blockade with DPCPX. Both P2X(1) and A(1) receptor stimulation with alpha,beta-methylene ATP and CPA, respectively, caused dose-dependent decreases in RBF. Administration of either PPADS or IP5I significantly blocked P2X(1) receptor stimulation. Likewise, administration of DPCPX significantly blocked A(1) receptor activation to CPA. Autoregulatory behavior was assessed by measuring RBF responses to reductions in renal perfusion pressure. In vehicle-infused rats, as pressure was decreased from 120 to 100 mmHg, there was no decrease in RBF. However, in either PPADS- or IP5I-infused rats, each decrease in pressure resulted in a significant decrease in RBF, demonstrating loss of autoregulatory ability. In DPCPX-infused rats, reductions in pressure did not cause significant reductions in RBF over the pressure range of 100-120 mmHg, but the autoregulatory curve tended to be steeper than vehicle-infused rats over the range of 80-100 mmHg, suggesting that A(1) receptors may influence RBF at lower pressures. These findings are consistent with in vitro data from afferent arterioles and support the hypothesis that P2X(1) receptor activation is important for whole kidney autoregulation in vivo.
File Format	HTM / HTML
ISSN	1931857X
e-ISSN	15221466
DOI	10.1152/ajprenal.00016.2010
Journal	AJP: Renal Physiology
Issue Number	6
Volume Number	298
Language	English
Publisher	American Physiological Society
Publisher Date	2010-06-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Adenosine A1 Receptor Agonists Blood Pressure Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't Blood Supply Arterioles Homeostasis Adenosine Triphosphate Kidney Receptors, Purinergic P2x Discipline Physiology Dose-response Relationship, Drug Adenosine Receptors, Purinergic P2 Adenosine A1 Receptor Antagonists Purinergic P2 Receptor Antagonists Rats, Sprague-dawley Discipline Nephrology Pharmacology Purinergic P2 Receptor Agonists Metabolism Drug Effects Pyridoxal Phosphate Xanthines Animals Renal Circulation Receptor, Adenosine A1 Dinucleoside Phosphates Analogs & Derivatives
Content Type	Text
Resource Type	Article
Subject	Physiology Urology

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