NDLI: Role of spinal metabotropic glutamate receptor 5 in pudendal inhibition of the nociceptive bladder reflex in cats.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Reese, Jeremy N. Rogers, Marc J. Xiao, Zhiying Shen, Bing Wang, Jicheng Schwen, Zeyad Roppolo, James R. de Groat, William C. Tai, Changfeng
Description	Author Affiliation: Reese JN ( Department of Urology, University of Pittsburgh, Pittsburgh, Pennsylvania); Rogers MJ ( Department of Urology, University of Pittsburgh, Pittsburgh, Pennsylvania); Xiao Z ( Department of Urology, University of Pittsburgh, Pittsburgh, Pennsylvania); Shen B ( Department of Urology, University of Pittsburgh, Pittsburgh, Pennsylvania); Wang J ( Department of Urology, University of Pittsburgh, Pittsburgh, Pennsylvania); Schwen Z ( Department of Urology, University of Pittsburgh, Pittsburgh, Pennsylvania); Roppolo JR ( Department of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, Pennsylvania.); de Groat WC ( Department of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, Pennsylvania.); Tai C ( Department of Urology, University of Pittsburgh, Pittsburgh, Pennsylvania)
Abstract	This study examined the role of spinal metabotropic glutamate receptor 5 (mGluR5) in the nociceptive C-fiber afferent-mediated spinal bladder reflex and in the inhibtion of this reflex by pudendal nerve stimulation (PNS). In -chloralose-anesthetized cats after spinal cord transection at the T9/T10 level, intravesical infusion of 0.25% acetic acid irritated the bladder, activated nociceptive C-fiber afferents, and induced spinal reflex bladder contractions of low amplitude (<50 cmH2O) and short duration (<20 s) at a smaller bladder capacity â¼80% of saline control capacity. PNS significantly (P < 0.01) increased bladder capacity from 85.5 ± 10.1 to 137.3 ± 14.1 or 148.2 ± 11.2% at 2T or 4T stimulation, respectively, where T is the threshold intensity for PNS to induce anal twitch. MTEP {3-[(2-methyl-4-thiazolyl)ethynyl]pyridine; 3 mg/kg iv, a selective mGluR5 antagonist} completely removed the PNS inhibition and significantly (P < 0.05) increased bladder capacity from 71.8 ± 9.9 to 94.0 ± 13.9% of saline control, but it did not change the bladder contraction amplitude. After propranolol (3 mg/kg iv, a ß1/ß2-adrenergic receptor antagonist) treatment, PNS inhibition remained but MTEP significantly (P < 0.05) reduced the bladder contraction amplitude from 18.6 ± 2.1 to 6.6 ± 1.2 cmH2O and eliminated PNS inhibition. At the end of experiments, hexamethonium (10 mg/kg iv, a ganglionic blocker) significantly (P < 0.05) reduced the bladder contraction amplitude from 20.9 ± 3.2 to 8.1 ± 1.5 cmH2O on average demonstrating that spinal reflexes were responsible for a major component of the contractions. This study shows that spinal mGluR5 plays an important role in the nociceptive C-fiber afferent-mediated spinal bladder reflex and in pudendal inhibition of this spinal reflex.
File Format	HTM / HTML
ISSN	1931857X
e-ISSN	15221466
DOI	10.1152/ajprenal.00623.2014
Journal	AJP: Renal Physiology
Issue Number	8
Volume Number	308
Language	English
Publisher	American Physiological Society
Publisher Date	2015-04-15
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Research Support, N.i.h., Extramural Therapy Neural Inhibition Antagonists & Inhibitors Action Potentials Discipline Physiology Acetic Acid Time Factors Thiazoles Receptor, Metabotropic Glutamate 5 Spinal Nerves Disease Models, Animal Nociception Pyridines Signal Transduction Discipline Nephrology Pharmacology Metabolism Nerve Fibers, Unmyelinated Drug Effects Chemically Induced Physiopathology Muscle, Smooth Urodynamics Pudendal Nerve Urinary Bladder, Overactive Animals Muscle Contraction Spinal Cord Injuries Thoracic Vertebrae Nociceptors Urinary Bladder Excitatory Amino Acid Antagonists
Content Type	Text
Resource Type	Article
Subject	Physiology Urology

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