| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Fan, Fan Chen, Chun Cheng Andy Zhang, Jin Schreck, Carlos M. N. Roman, Eric A. Williams, Jan M. Hirata, Takashi Sharma, Mukut Beard, Daniel A. Savin, Virginia J. Roman, Richard J. |
| Description |
Author Affiliation: Fan F ( Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson, Mississippi); Chen CC ( Department of Pharmacology, Lake Erie College of Osteopathic Medicine, Erie, Pennsylvania); Zhang J ( Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson, Mississippi); Schreck CM ( Department of Nephrology, Hospital Italiano de Buenos Aires, Buenos Aires, Argentina); Roman EA ( Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson, Mississippi); Williams JM ( Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson, Mississippi); Hirata T ( Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson, Mississippi); Sharma M ( Department of Nephrology, Internal Medicine, Kansas City Veterans Affairs Medical Center, Kansas City, Missouri); Beard DA ( Department of Molecular and Integrative Physiology, Internal Medicine, University of Michigan, Ann Arbor, Michigan.); Savin VJ ( Department of Nephrology, Internal Medicine, Kansas City Veterans Affairs Medical Center, Kansas City, Missouri); Roman RJ ( Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson, Mississippi) |
| Abstract |
This study describes a high-throughput fluorescence dilution technique to measure the albumin reflection coefficient (σAlb) of isolated glomeruli. Rats were injected with FITC-dextran 250 (75 mg/kg), and the glomeruli were isolated in a 6% BSA solution. Changes in the fluorescence of the glomerulus due to water influx in response to an imposed oncotic gradient was used to determine σAlb. Adjustment of the albumin concentration of the bath from 6 to 5, 4, 3, and 2% produced a 10, 25, 35, and 50% decrease in the fluorescence of the glomeruli. Pretreatment of glomeruli with protamine sulfate (2 mg/ml) or TGF-ß1 (10 ng/ml) decreased σAlb from 1 to 0.54 and 0.48, respectively. Water and solute movement were modeled using Kedem-Katchalsky equations, and the measured responses closely fit the predicted behavior, indicating that loss of albumin by solvent drag or diffusion is negligible compared with the movement of water. We also found that σAlb was reduced by 17% in fawn hooded hypertensive rats, 33% in hypertensive Dahl salt-sensitive (SS) rats, 26% in streptozotocin-treated diabetic Dahl SS rats, and 21% in 6-mo old type II diabetic nephropathy rats relative to control Sprague-Dawley rats. The changes in glomerular permeability to albumin were correlated with the degree of proteinuria in these strains. These findings indicate that the fluorescence dilution technique can be used to measure σAlb in populations of isolated glomeruli and provides a means to assess the development of glomerular injury in hypertensive and diabetic models. |
| File Format |
HTM / HTML |
| ISSN |
1931857X |
| Issue Number |
12 |
| Volume Number |
309 |
| e-ISSN |
15221466 |
| Journal |
AJP: Renal Physiology |
| Language |
English |
| Publisher |
American Physiological Society |
| Publisher Date |
2015-12-15 |
| Publisher Place |
United States |
| Access Restriction |
Subscribed |
| Subject Keyword |
Urine Research Support, N.i.h., Extramural Diabetic Nephropathies Streptozocin Mice, Inbred C57bl Fluorescence Rats, Sprague-dawley Discipline Nephrology Permeability Metabolism Drug Effects Chemically Induced Journal Article Physiopathology Discipline Physiology Albumins Animals Analysis Diabetes Mellitus, Experimental Rats, Inbred Dahl Indicator Dilution Techniques Kidney Glomerulus Proteinuria |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Physiology Urology |
