NDLI: De novo transcriptome sequencing in Pueraria lobata to identify putative genes involved in isoflavones biosynthesis.

Please wait, while we are loading the content...

Year: 2017 Volume: 36

Year: 2016 Volume: 35

Year: 2015 Volume: 34

Year: 2015 Volume: 34 Issue: 12

Year: 2015 Volume: 34 Issue: 11

Year: 2015 Volume: 34 Issue: 10

Year: 2015 Volume: 34 Issue: 9

Year: 2015 Volume: 34 Issue: 8

Year: 2015 Volume: 34 Issue: 7

Year: 2015 Volume: 34 Issue: 6

Year: 2015 Volume: 34 Issue: 5

Transgenic rice expressing a codon-modified synthetic CP4-EPSPS confers tolerance to broad-spectrum herbicide, glyphosate.

De novo transcriptome sequencing in Pueraria lobata to identify putative genes involved in isoflavones biosynthesis.

Effect of Agrobacterium strain and plasmid copy number on transformation frequency, event quality and usable event quality in an elite maize cultivar.

Responses of rose RhACS1 and RhACS2 promoters to abiotic stresses in transgenic Arabidopsis thaliana.

Somatic embryogenesis in ferns: a new experimental system.

TaUBA, a UBA domain-containing protein in wheat (Triticum aestivum L.), is a negative regulator of salt and drought stress response in transgenic Arabidopsis.

Alteration of osa-miR156e expression affects rice plant architecture and strigolactones (SLs) pathway.

Identification of genes that may regulate the expression of the transcription factor production of anthocyanin pigment 1 (PAP1)/MYB75 involved in Arabidopsis anthocyanin biosynthesis.

PtrWRKY73, a salicylic acid-inducible poplar WRKY transcription factor, is involved in disease resistance in Arabidopsis thaliana.

Overexpression of LOV KELCH protein 2 confers dehydration tolerance and is associated with enhanced expression of dehydration-inducible genes in Arabidopsis thaliana.

Visualisation of plastid outgrowths in potato (Solanum tuberosum L.) tubers by carboxyfluorescein diacetate staining.

A red fluorescent protein (DsRED) from Discosoma sp. as a reporter for gene expression in walnut somatic embryos.

A GSHS-like gene from Lycium chinense maybe regulated by cadmium-induced endogenous salicylic acid and overexpression of this gene enhances tolerance to cadmium stress in Arabidopsis.

Chalcone synthase EaCHS1 from Eupatorium adenophorum functions in salt stress tolerance in tobacco.

Arabidopsis thaliana defense response to the ochratoxin A-producing strain (Aspergillus ochraceus 3.4412).

Isolation and characterization of a J domain protein that interacts with ARC1 from ornamental kale (Brassica oleracea var. acephala).

Improved nitrogen use efficiency in transgenic sugarcane: phenotypic assessment in a pot trial under low nitrogen conditions.

Year: 2015 Volume: 34 Issue: 4

Year: 2015 Volume: 34 Issue: 3

Year: 2015 Volume: 34 Issue: 2

Year: 2015 Volume: 34 Issue: 1

Year: 2014 Volume: 33

Year: 2013 Volume: 32

Year: 2012 Volume: 31

Year: 2011 Volume: 30

Year: 2010 Volume: 29

Year: 2009 Volume: 28

Year: 2008 Volume: 27

Year: 2007 Volume: 26

Year: 1996 Volume: 16

Year: 1996 Volume: 15

Year: 1995 Volume: 15

Year: 1995 Volume: 14

Year: 1994 Volume: 14

Year: 1994 Volume: 13

Year: 1993 Volume: 13

Year: 1993 Volume: 12

Year: 1992 Volume: 12

Year: 1992 Volume: 11

Year: 1992 Volume: 10

Year: 1991 Volume: 10

Year: 1991 Volume: 9

Year: 1990 Volume: 9

Year: 1990 Volume: 8

Year: 1989 Volume: 8

Year: 1989 Volume: 7

Year: 1988 Volume: 7

Year: 1987 Volume: 6

Year: 1986 Volume: 5

Year: 1985 Volume: 4

Year: 1984 Volume: 3

Year: 1983 Volume: 2

Year: 1982 Volume: 1

Year: 1981 Volume: 1

De novo transcriptome sequencing in Pueraria lobata to identify putative genes involved in isoflavones biosynthesis.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Wang, Xin Li, Shutao Li, Jia Li, Changfu Zhang, Yansheng
Description	Country affiliation: China Author Affiliation: Wang X ( CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei, China.)
Abstract	KEY MESSAGE: Using Illumina sequencing technology, we have generated the large-scale transcriptome sequencing data and indentified many putative genes involved in isoflavones biosynthesis in Pueraria lobata. Pueraria lobata, a member of the Leguminosae family, is a traditional Chinese herb which has been used since ancient times. P. lobata root has extensive clinical usages, because it contains a rich source of isoflavones, including daidzin and puerarin. However, the knowledge of isoflavone metabolism and the characterization of corresponding genes in such a pathway remain largely unknown. In this study, de novo transcriptome of P. lobata root and leaf was sequenced using the Solexa sequencing platform. Over 140 million high-quality reads were assembled into 163,625 unigenes, of which about 43.1% were aligned to the Nr protein database. Using the RPKM (reads per kilo bases per million reads) method, 3,148 unigenes were found to be upregulated, and 2,011 genes were downregulated in the leaf as compared to those in the root. Towards a further understanding of these differentially expressed genes, Gene ontology enrichment and metabolic pathway enrichment analyses were performed. Based on these results, 47 novel structural genes were identified in the biosynthesis of isoflavones. Also, 22 putative UDP glycosyltransferases and 45 O-methyltransferases unigenes were identified as the candidates most likely to be involved in the tailoring processes of isoflavonoid downstream pathway. Moreover, MYB transcription factors were analyzed, and 133 of them were found to have higher expression levels in the roots than in the leaves. In conclusion, the de novo transcriptome investigation of these unique transcripts provided an invaluable resource for the global discovery of functional genes related to isoflavones biosynthesis in P. lobata.
File Format	HTM / HTML
ISSN	07217714
Issue Number	5
Volume Number	34
e-ISSN	1432203X
Journal	Plant Cell Reports
Language	English
Publisher	Springer
Publisher Date	2015-05-01
Publisher Place	Germany
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Botany Isoflavones Metabolism Plant Proteins Genetics Pueraria Transcriptome Biosynthetic Pathways Dna, Complementary Chemistry Dna, Plant Gene Expression Profiling Gene Ontology Glycosyltransferases Classification High-throughput Nucleotide Sequencing Methyltransferases Molecular Sequence Data Plant Leaves Plant Roots Sequence Analysis, Dna Comparative Study Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Plant Science Medicine Agronomy and Crop Science

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in