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Optimization of process parameters for supercritical fluid extraction of cholesterol from whole milk powder using ethanol as co-solvent.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Dey Paul, Indira Jayakumar, Chitra Niwas Mishra, Hari
Description	Country affiliation: India Author Affiliation: Dey Paul I ( Agricultural and Food Engineering Department, Indian Institute of Technology Kharagpur, West Bengal, 721302, India. indira27dp@gmail.com.); Jayakumar C ( Agricultural and Food Engineering Department, Indian Institute of Technology Kharagpur, West Bengal, 721302, India.); Niwas Mishra H ( Agricultural and Food Engineering Department, Indian Institute of Technology Kharagpur, West Bengal, 721302, India.)
Abstract	BACKGROUND: In spite of being highly nutritious, the consumption of milk is hindered because of its high cholesterol content, which is responsible for numerous cardiac diseases. Supercritical carbon dioxide using ethanol as co-solvent was employed to extract cholesterol from whole milk powder (WMP). This study was undertaken to optimize the process parameters of supercritical fluid extraction (SCFE), viz. extraction temperature, pressure and volume of ethanol. RESULTS: The cholesterol content of WMP was quantified using high-performance liquid chromatography. The impact of the extraction conditions on the fat content (FC), solubility index (SI) and lightness (L) of the SCFE-treated WMP were also investigated. The process parameters were optimized using response surface methodology. About 46% reduction in cholesterol was achieved at the optimized conditions of 48 °C, 17 MPa and 31 mL co-solvent; flow rate of expanded CO , static time and dynamic time of extraction were 6 L min , 10 min and 80 min respectively. The treated WMP retained its FC, SI, and L at moderate limits of 183.67 g kg , 96.3% and 96.90, respectively. CONCLUSION: This study demonstrated the feasibility of ethanol-modified SCFE of cholesterol from WMP with negligible changes in its physicochemical properties. © 2016 Society of Chemical Industry.
File Format	HTM / HTML
ISSN	00225142
Issue Number	15
Journal	Journal of the Science of Food and Agriculture
Volume Number	96
e-ISSN	10970010
Language	English
Publisher	Wiley
Publisher Date	2016-12-01
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Nutrition and Dietetics Food Science Biotechnology Agronomy and Crop Science

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