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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Ebanks, Jody P. Koshoffer, Amy Wickett, R. Randall Schwemberger, Sandy Babcock, George Hakozaki, Tomohiro Boissy, Raymond E. |
| Description | Country affiliation: United States Author Affiliation: Ebanks JP ( College of Pharmacy, University of Cincinnati, Cincinnati, Ohio, USA.) |
| Abstract | Modification of skin complexion coloration has traditionally been accomplished by interruption or attenuation of melanogenesis and/or melanosome transfer. Post-transfer modification of pigmented melanosomes provides an attractive and distinct avenue of modulating skin pigmentation. The processing of melanosomes during keratinocyte (KC) terminal differentiation and the degradative variability observed between light and dark skin (LS and DS) remains enigmatic. To evaluate this, we developed a model system to investigate the loss of fluorescently labeled and isolated melanosomes by cultured human KCs. The extent of melanosome loss has been qualitatively assessed using transmission electron microscopy and indirect immunofluorescence with confocal microscopy, and quantitatively assessed using flow cytometry analysis. Results show that melanosomes are incorporated into the cytoplasm of both light and dark keratinocytes (LKCs and DKCs) and trafficked to a perinuclear region. Within 48 hours, confocal microscopy images suggest that LKCs display accelerated melanosome loss. This time-dependent decrease in carboxyfluorescein diacetate (CFDA) fluorescence was then quantitatively analyzed using flow cytometry. Consistent with the results of the confocal analysis, over a 48-hour time frame, LKCs appear to lose melanosomes more efficiently than DKCs. These experiments show that melanosomes are more rapidly lost in KCs derived from LS as opposed to DS. |
| File Format | HTM / HTML |
| ISSN | 0022202X |
| e-ISSN | 15231747 |
| Journal | Journal of Investigative Dermatology |
| Issue Number | 6 |
| Volume Number | 131 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2011-06-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Discipline Dermatology Epidermis Cytology Keratinocytes Ultrastructure Melanosomes Skin Pigmentation Cells, Cultured Flow Cytometry Fluorescent Antibody Technique, Indirect Microscopy, Confocal Microscopy, Electron, Transmission Comparative Study Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry Molecular Biology Dermatology |
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