NDLI: Fibronectin expression determines skin cell motile behavior.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Hamill, Kevin J. Hopkinson, Susan B. Hoover, Paul Todorovic, Viktor Green, Kathleen J. Jones, Jonathan C. R.
Description	Country affiliation: United States Author Affiliation: Hamill KJ ( Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA.)
Abstract	Mouse keratinocytes migrate significantly slower than their human counterparts in vitro on uncoated surfaces. We tested the hypothesis that this is a consequence of differences in the extracellular matrix (ECM) that cells deposit. In support of this, human keratinocyte motility was markedly reduced when plated onto the ECM of mouse skin cells, whereas the latter cells migrated faster when plated onto human keratinocyte ECM. The ECM of mouse and human keratinocytes contained similar levels of the 3 laminin subunit of laminin-332. However, mouse skin cells expressed significantly more fibronectin (FN) than human cells. To assess whether FN is a motility regulator, we used small interfering RNA (siRNA) to reduce the expression of FN in mouse keratinocytes. The treated mouse keratinocytes moved significantly more rapidly than wild-type mouse skin cells. Moreover, the FN-depleted mouse cell ECM supported increased migration of both mouse and human keratinocytes. Furthermore, the motility of human keratinocytes was slowed when plated onto FN-coated substrates or human keratinocyte ECM supplemented with FN in a dose-dependent manner. Consistent with these findings, the ECM of 3 integrin-null keratinocytes, which also migrated faster than wild-type cells, was FN deficient. Our results provide evidence that FN is a brake to skin cell migration supported by laminin-332-rich matrices.
File Format	HTM / HTML
ISSN	0022202X
e-ISSN	15231747
DOI	10.1038/jid.2011.297
Journal	Journal of Investigative Dermatology
Issue Number	2
Volume Number	132
Language	English
Publisher	Elsevier
Publisher Date	2012-02-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Dermatology Cell Movement Fibronectins Physiology Keratinocytes Animals Cell Adhesion Molecules Cell Line Integrin Alpha3 Mice Mice, Inbred C57bl Research Support, N.i.h., Extramural
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology Dermatology

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