| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Wang, Xiao-Nong McGovern, Naomi Gunawan, Merry Richardson, Connor Windebank, Martin Siah, Tee-Wei Lim, Hwee-Ying Fink, Katja Li, Jackson L. Yao Ng, Lai G. Ginhoux, Florent Angeli, Veronique Collin, Matthew Haniffa, Muzlifah |
| Description |
Country affiliation: United kingdom Author Affiliation: Wang XN ( Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK.); McGovern N ( Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK.); Gunawan M ( Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK.); Richardson C ( Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK.); Windebank M ( Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK.); Siah TW ( Department of Dermatology, Royal Victoria Infirmary, Newcastle upon Tyne, UK.); Lim HY ( National University of Singapore, Singapore, Singapore.); Fink K ( Singapore Immunology Network, A*Star, Singapore, Singapore.); Li JL ( Singapore Immunology Network, A*Star, Singapore, Singapore.); Ng LG ( Singapore Immunology Network, A*Star, Singapore, Singapore.); Ginhoux F ( Singapore Immunology Network, A*Star, Singapore, Singapore.); Angeli V ( National University of Singapore, Singapore, Singapore.); Collin M ( Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK.); Haniffa M ( Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK.) |
| Abstract |
Dendritic cells (DCs), macrophages (Mφ), and T cells are major components of the skin immune system, but their interstitial spatial organization is poorly characterized. Using four-channel whole-mount immunofluorescence staining of the human dermis, we demonstrated the three-dimensional distribution of CD31(+) blood capillaries, LYVE-1(+) lymphatics, discrete populations of CD11c(+) myeloid DCs, FXIIIa(+) Mφ, and lymphocytes. We showed phenotypic and morphological differences in situ between DCs and Mφ. DCs formed the first dermal cellular layer (0-20 µm beneath the dermoepidermal junction), Mφ were located deeper (40-60 µm), and CD3(+) lymphocytes were observed throughout (0-60 µm). Below this level, DCs, T cells, and the majority of Mφ formed stable perivascular sheaths. Whole-mount imaging revealed the true extent of dermal leukocytes previously underestimated from cross-section views. The total area of apical dermis (0-30 µm) contained approximately 10-fold more myeloid DCs than the entire blood volume of an average individual. Surprisingly, <1% of dermal DCs occupied lymphatics in freshly isolated skin. Dermal DCs rapidly accumulated within lymphatics, but Mφ remained fixed in skin explants cultured ex vivo. The leukocyte architecture observed in normal skin was distorted in inflammation and disease. These studies illustrate the micro-anatomy of dermal leukocytes and provide further insights into their functional organization. |
| File Format |
HTM / HTML |
| ISSN |
0022202X |
| e-ISSN |
15231747 |
| DOI |
10.1038/jid.2013.481 |
| Journal |
Journal of Investigative Dermatology |
| Issue Number |
4 |
| Volume Number |
134 |
| Language |
English |
| Publisher |
Elsevier |
| Publisher Date |
2014-04-01 |
| Publisher Place |
United States |
| Access Restriction |
Open |
| Subject Keyword |
Discipline Dermatology Leukocytes Cytology Macrophages Pathology Skin Blood Supply Adolescent Anisotropy Antigens, Cd11c Metabolism Antigens, Cd31 Carrier Proteins Cell Movement Dermis Flow Cytometry Langerhans Cells Lymphatic System Lymphatic Vessels Lymphocytes Lymphoma, T-cell, Cutaneous Microfilament Proteins Phenotype Research Support, Non-u.s. Gov't |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Cell Biology Biochemistry Molecular Biology Dermatology |
