NDLI: Antioxidant effects of flavonoids used as food additives (purple corn color, enzymatically modified isoquercitrin, and isoquercitrin) on liver carcinogenesis in a rat medium-term bioassay.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Yokohira, M. Yamakawa, K. Saoo, K. Matsuda, Y. Hosokawa, K. Hashimoto, N. Kuno, T. Imaida, K.
Description	Country affiliation: Japan Author Affiliation: Yokohira M ( Dept of Pathology and Host-Defence, Faculty of Medicine, Kagawa Univ, Kita-gun, Kagawa, Japan.)
Abstract	To clarify the effects of purple corn color, enzymatically modified isoquercitrin (EMIQ), and isoquercitrin (IQ), registered as natural food additives in Japan, on liver carcinogenesis in vivo, a medium-term bioassay was employed. A total of 100 male F344 rats were divided into 5 groups; groups 1 to 4 were given a single intraperitoneal injection of diethylnitrosamine (200 mg/kg b.w.) on day 1. From weeks 2 to 8, they were administered basal diet purple corn color, EMIQ, or IQ as containing test chemicals at doses of 1.0% (groups 1 and 5), 0.1% (group 2), 0.01% (group 3), or 0% (group 4) (experiments 1, 4, and 5). All rats were subjected to two-thirds partial hepatectomy at week 3 and were sacrificed at week 8. Purple corn color exerted no significant modifying effects on GST-P positive foci, preneoplastic foci, development in the liver. However, serum of rats treated with purple corn color provided evidence of antioxidant power significantly by potential antioxidant (PAO) test in vivo (experiment 2). And microarray analyses showed purple corn color to induce RNA expression such as P450 (cytochrome) oxidoreductase, phosphatidylinositol 3-kinase, and phospholipase A2 (experiment 3). Higher doses of EMIQ or IQ with strong antioxidant power in vivo by PAO test treated groups were correlated with smaller numbers of GST-P positive foci, with Spearman's rank correlation coefficients of P= 0.002 and P= 0.049, respectively (experiments 4 and 5). Therefore, the tested food additives may be effective as antioxidants in vivo and have chemopreventive potential against liver preneoplastic lesion development.
File Format	HTM / HTML
ISSN	00221147
Issue Number	7
Volume Number	73
e-ISSN	17503841
Journal	Journal of Food Science
Language	English
Publisher	Wiley (on behalf ofInstitute of Food Technologists)
Publisher Date	2008-09-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Food science Anticarcinogenic Agents Pharmacology Antioxidants Flavonoids Food Additives Liver Neoplasms, Experimental Prevention & Control Quercetin Analogs & Derivatives Animals Anthocyanins Diethylnitrosamine Toxicity Gene Expression Glutathione Transferase Metabolism Genetics Male Oligonucleotide Array Sequence Analysis Rna Rats Rats, Inbred F344 Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Food Science

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