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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Stevens, Jasper Van Den Berg, Dirk-jan de Ridder, Sanne Niederländer, Harm A. G. Van Der Graaf, Piet Hein Danhof, Meindert de Lange, Elizabeth C. M. |
| Description | Country affiliation: Netherlands Author Affiliation: Stevens J ( Division of Pharmacology, LACDR Leiden University, Leiden, The Netherlands.) |
| Abstract | Remoxipride is a selective dopamine D(2) receptor antagonist, and useful as a model compound in mechanism-based pharmacological investigations. To that end, studies in small animals with serial sampling over time are needed. For these small volume samples currently no suitable analytical methods are available. We propose analytical methods for the detection of low concentrations remoxipride in small sample volumes of plasma, brain homogenate, and brain microdialysate, using online solid phase extraction with liquid chromatography-tandem mass spectrometry. Method development, optimization and validation are described in terms of calibration curves, extraction yield, lower limit of quantification (LLOQ), precision, accuracy, inter-day- and intra-day variability. The 20 microl plasma samples showed an extraction yield of 76%, with a LLOQ of 0.5 ng/ml. For 0.6 ml brain homogenate samples the extraction yield was 45%, with a LLOQ of 1.8 ng/ml. The 20 microl brain microdialysate samples, without pre-treatment, had a LLOQ of 0.25 ng/ml. The precision and accuracy were well within the acceptable 15% range. Considering the small sample volumes, the high sensitivity and good reproducibility, the analytical methods are suitable for analyzing small sample volumes with low remoxipride concentrations. |
| File Format | HTM / HTML |
| ISSN | 15700232 |
| Issue Number | 13-14 |
| Volume Number | 878 |
| e-ISSN | 1873376X |
| Journal | Journal of Chromatography B |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2010-04-15 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Chemistry Brain Metabolism Chromatography, Liquid Methods Dopamine Antagonists Pharmacokinetics Remoxipride Tandem Mass Spectrometry Calibration Blood Limit Of Detection Microdialysis Reproducibility Of Results Journal Article Research Support, Non-u.s. Gov't Validation Studies |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine Analytical Chemistry Clinical Biochemistry Biochemistry |
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