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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Serve, Kinta M. Darnell, Jennifer L. Takemoto, Jody K. Davies, Neal M. Black, Margaret E. |
| Description | Country affiliation: United States Author Affiliation: Serve KM ( School of Molecular Biosciences, Washington State University, Pullman, WA 99164, USA.) |
| Abstract | The efficacy of the chemotherapeutic drug 5'-fluorouracil is reduced by catabolism to 2'-fluoro-beta-alanine (FBAL), a three-step reaction in which dihydropyrimidine dehydrogenase (DPD) catalyzes the rate-limiting step. To study in vitro DPD activity, we developed and validated an isocratic, reverse-phase HPLC method to detect and quantify FBAL without using multiple columns or radiolabeled substrates. Pre-column derivatization of FBAL was performed using o-phthalaldehyde in the presence of two sulfur donors, ethanthiol or beta-mercaptoethanol, and the resulting products assayed. Calibration curves were linear over a range of 10-200 microg/ml and the method was successfully applied to the examination of DPD activity in cultured cells. |
| File Format | HTM / HTML |
| ISSN | 15700232 |
| e-ISSN | 1873376X |
| DOI | 10.1016/j.jchromb.2010.05.010 |
| Journal | Journal of Chromatography B |
| Issue Number | 21 |
| Volume Number | 878 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2010-07-01 |
| Publisher Place | Netherlands |
| Access Restriction | Open |
| Subject Keyword | Discipline Analytical Chemistry Chromatography, High Pressure Liquid Dihydrouracil Dehydrogenase (nadp) Metabolism Beta-alanine Analogs & Derivatives Cell Line, Tumor Chromatography, Reverse-phase Drug Stability Fluorouracil Reproducibility Of Results Sensitivity And Specificity Sulfhydryl Compounds Chemistry O-phthalaldehyde Research Support, N.i.h., Extramural |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine Analytical Chemistry Clinical Biochemistry Biochemistry |
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