NDLI: Parallel sample preparation of proteins, from crude samples to crystals ready for MALDI-MS, in an integrated microfluidic system.

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Parallel sample preparation of proteins, from crude samples to crystals ready for MALDI-MS, in an integrated microfluidic system.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Tran, Thi Thuy Inganäs, Mats Ekstrand, Gunnar Thorsén, Gunnar
Description	Country affiliation: Sweden Author Affiliation: Tran TT ( Department of Analytical Chemistry, Stockholm University, SE-106 91 Stockholm, Sweden.)
Abstract	A microfluidic structure is presented where selective capture of proteins in complex samples, followed by clean-up, enzymatic processing, and MALDI-MS sample preparation of peptides generated, can be performed. The structure uses an affinity column to capture the protein while all other components in the sample are disposed of. The protein of interest is then eluted from the affinity column and captured on a second column on which the enzymatic processing is performed. Salts and hydrophilic contaminants are then removed before the products from the enzymatic reaction are eluted together with a suitable MALDI matrix and the solvent evaporated in a designated MALDI target structure. All steps can be performed automatically in 54 parallel microstructures on a microfluidic compact disc. The process is demonstrated by the selective capture and tryptic digest of recombinant IgG molecules from samples containing other proteins: an excess of bovine serum albumin or spent cell culture media.
File Format	HTM / HTML
ISSN	15700232
Issue Number	28
Volume Number	878
e-ISSN	1873376X
Journal	Journal of Chromatography B
Language	English
Publisher	Elsevier
Publisher Date	2010-10-15
Publisher Place	Netherlands
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Analytical Chemistry Microfluidic Analytical Techniques Instrumentation Methods Peptide Mapping Recombinant Proteins Isolation & Purification Spectrometry, Mass, Matrix-assisted Laser Desorption-ionization Animals Cattle Humans Immunoglobulin G Metabolism Spectrometry, Fluorescence Trypsin Journal Article
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine Analytical Chemistry Clinical Biochemistry Biochemistry

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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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