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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Kang, Seung Woo Jang, Hae Jong Moore, Victor S. Park, Ji-Young Kim, Kyoung-Ah Youm, Jeong-Rok Han, Sang Beom |
| Description | Author Affiliation: Kang SW ( Department of Analytical Research Service, International Scientific Standards Ltd. A Battelle Company, Chuncheon 200-161, Republic of Korea.) |
| Abstract | A highly sensitive and enantioselective method has been developed and validated for the determination of levocetirizine [(R)-cetirizine] in human plasma by normal-phase liquid chromatography coupled to tandem mass spectrometry with an atmospheric pressure chemical ionization (APCI) interface in the positive ion mode. Enantioselective separation was achieved on a CHIRALPAK AD-H column using an isocratic mobile phase consisting of a mixture of n-hexane, ethyl alcohol, diethylamine, and acetic acid (60:40:0.1:0.1, v/v/v/v). Levocetirizine-D(8) was used as an internal standard (IS). Levocetirizine and the IS were detected by multiple-reaction monitoring (MRM). Mass transitions of analyte and IS were m/z 389.2â201.1 and 397.2â201.1, respectively. Under optimized analytical conditions, a baseline separation of two enantiomers and IS was obtained in less than 11 min. Samples were prepared by a simple two-step extraction by protein precipitation using acetonitrile followed by liquid-liquid extraction with a n-hexane-dichloromethane mixture (50:50, v/v). The standard curve for levocetirizine was linear (r(2)>0.995) in the concentration range 0.5-300 ng/mL. Recovery was between 97.0 and 102.2% at low, medium, and high concentration. The limit of quantification (LOQ) was 0.5 ng/mL. Other method validation parameters, such as precision, accuracy, and stability, were very satisfactory. Finally, the proposed method was successfully applied to the study of enantioselective oral pharmacokinetics of levocetirizine in healthy Korean volunteers. |
| File Format | HTM / HTML |
| ISSN | 15700232 |
| Issue Number | 32 |
| Volume Number | 878 |
| e-ISSN | 1873376X |
| Journal | Journal of Chromatography B |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2010-12-15 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Chemistry Cetirizine Blood Chromatography, High Pressure Liquid Methods Histamine H1 Antagonists, Non-sedating Tandem Mass Spectrometry Chemistry Pharmacokinetics Drug Stability Humans Linear Models Reproducibility Of Results Sensitivity And Specificity Stereoisomerism Journal Article |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine Analytical Chemistry Clinical Biochemistry Biochemistry |
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