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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Toueille, Magali Uzel, Audrey Depoisier, Jean-François Gantier, René |
| Description | Country affiliation: France Author Affiliation: Toueille M ( Pall Life Sciences, Cergy St Christophe, France.) |
| Abstract | Current platforms for purification of monoclonal antibodies, mostly relying on Protein A as a first capture step, are robust and efficient but significantly increase downstream purification costs, mainly due to Protein A resins. To decrease manufacturing costs, industry is increasingly considering the use of purification schemes without affinity Protein A resins. Mixed-mode chromatography can be used as a powerful alternative to standard purification platforms as it offers new selectivity and separation mechanisms exploiting a combination of both ionic and hydrophobic characteristics of antibodies and contaminating proteins. By using a design of experiments (DoE) approach and high throughput screening in 96-well plates, we developed four different two-steps MAb purification processes, based on the use of mixed-mode sorbents. Finally, three of the tested processes resulted in final purified Mab fractions containing less than 100 ppm of residual CHO proteins (CHOP), with overall process yields above 70%. These data show that mixed-mode chromatography sorbents, used at capture or intermediate purification steps, really expand the options of MAb purification process development with or without Protein A affinity chromatography. |
| File Format | HTM / HTML |
| ISSN | 15700232 |
| Issue Number | 13-14 |
| Volume Number | 879 |
| e-ISSN | 1873376X |
| Journal | Journal of Chromatography B |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2011-04-15 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Chemistry Antibodies, Monoclonal Isolation & Purification Chromatography, Affinity Methods High-throughput Screening Assays Adsorption Animals Chemistry Cho Cells Instrumentation Computational Biology Cricetinae Cricetulus Staphylococcal Protein A Journal Article |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine Analytical Chemistry Clinical Biochemistry Biochemistry |
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