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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Kvaskoff, David Ko, Pauline Simila, Henry A. Eyles, Darryl W. |
| Description | Country affiliation: Australia Author Affiliation: Kvaskoff D ( Queensland Brain Institute, The University of Queensland, Brisbane, Queensland 4072, Australia.) |
| Abstract | Dried blood spots (DBS) are a convenient collection and archiving method for blood specimens. The interest in screening certain analytes in neonatal DBS continues to increase for a variety of paediatric disorders. 25-Hydroxyvitamin D(3) (25OHD(3)) is one such analyte. We investigated potential factors that may affect the analysis of 25OHD(3) in prospective cohorts of DBS, such as blood spot volume, hole punch position, and paper type. All of these factors were shown to affect 25OHD(3) levels measured. When blood volumes of <50 µL were spotted, 25OHD(3) concentrations extracted were significantly lower (P<0.0001). We also observed a chromatographic effect across the surface of blood spots, with 25OHD(3) concentrations significantly higher in outer punched spots compared to those punched from the centre (P<0.0001). This also correlates with a heavier net weight of blood from outer punched spots (P<0.0001). This effect was reproducible on two types of paper cards (Whatman 903(®) and FTA(®)), and paper type was shown to be highly relevant. We also show that the distribution of 25OHD(3) in whole blood is essentially extracellular, with over 98% of 25OHD(3) residing in the serum component. This may potentially explain why the diffusion properties of blood and type of chromatographic paper may significantly influence the distribution of such analytes in DBS. These factors should be taken into consideration for the prospective collection of DBS and analysis of 25OHD(3) in DBS. |
| File Format | HTM / HTML |
| ISSN | 15700232 |
| Volume Number | 901 |
| e-ISSN | 1873376X |
| Journal | Journal of Chromatography B |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2012-07-15 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Chemistry Calcifediol Blood Dried Blood Spot Testing Methods Tandem Mass Spectrometry Adult Standards Ethanol Humans Paper Prospective Studies Reproducibility Of Results Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine Analytical Chemistry Clinical Biochemistry Biochemistry |
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