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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | de Paiva, Maria José Nunes Menezes, Helvécio Costa Christo, Paulo Pereira Resende, Rodrigo Ribeiro Cardeal, Zenilda de Lourdes |
| Description | Country affiliation: Brazil Author Affiliation: de Paiva MJ ( Departamento de Química, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Belo Horizonte, MG 31270901, Brazil.) |
| Abstract | The determination of the concentrations of l-amino acids in cerebrospinal fluid (CSF) has been used to gain biochemical insight into central nervous system disorders. This paper describes a microwave-assisted derivatization (MAD) method using N,O-bis-(trimethylsilyl)trifluoroacetamide (BSTFA) as a derivatizing agent for determining the concentrations of l-amino acids in human CSF by gas chromatography with mass spectrometry (GC/MS). The experimental design used to optimize the conditions showed that the optimal derivatization time was 3min with a microwave power of 210W. The method showed good performance for the validation parameters. The sensitivity was very good, with limits of detection (LODs) ranging from 0.01µmolL(-1) to 4.24µmolL(-1) and limits of quantification (LOQs) ranging from 0.02 to 7.07µmolL(-1). The precision, measured using the relative standard deviation (RSD), ranged from 4.12 to 15.59% for intra-day analyses and from 6.36 to 18.71% for inter-day analyses. The coefficients of determination (R(2)) were above 0.990 for all amino acids. The optimized and validated method was applied to the determination of amino acid concentrations in human CSF. |
| File Format | HTM / HTML |
| ISSN | 15700232 |
| Volume Number | 931 |
| e-ISSN | 1873376X |
| Journal | Journal of Chromatography B |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2013-07-15 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Chemistry Amino Acids Cerebrospinal Fluid Chemical Fractionation Methods Gas Chromatography-mass Spectrometry Chemistry Isolation & Purification Humans Limit Of Detection Linear Models Microwaves Models, Biological Reproducibility Of Results Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine Analytical Chemistry Clinical Biochemistry Biochemistry |
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