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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Arafat, Tawfiq Arafat, Basil Awad, Riad Awwad, Ahmad Abu |
| Description | Author Affiliation: Arafat T ( University of Petra, Faculty of Pharmacy, Amman, Jordan. Electronic address: tarafat@uop.edu.jo.); Arafat B ( Al-Ahliyya Amman University, Faculty of Pharmacy and Medical Sciences, Amman, Jordan. Electronic address: b.arafat@ammanu.edu.jo.); awad R ( University of Petra, Faculty of Pharmacy, Amman, Jordan. Electronic address: r.awad@uop.edu.jo.); awwad AA ( Jordan Center for Pharmaceutical Research, P.O. Box 950435, Amman 11105, Jordan. Electronic address: ahmad_chem@hotmail.com.) |
| Abstract | A simple and sensitive liquid chromatography-tandem mass spectrometric method for quantification of loperamide in human plasma and saliva was developed and validated, and then successfully applied in pharmacokinetic clinical study to investigate and correlate bioavailability of Imodium(®) 2mg quartet tablet dose in both human plasma and saliva. Loperamide with labeled internal standard was extracted from its biological matrix by methanol as protein direct precipitant in single extraction step. Adequate chromatographic separation for analytes from plasma and saliva matrices was achieved using ACE C18 (50mm×2.1mm, 5µm) column, eluted by water/methanol/formic acid (30:70:0.1%, v/v), delivered isocratically at constant flow rate of 0.75ml/min. The method validation intends to investigate specificity, sensitivity, linearity, precision, accuracy, recovery, matrix effect and stability according to European guideline, and partial validation was applied on saliva, specificity, matrix effect, recovery, sensitivity, within and between day precision and accuracy. The calibration curve was linear through the range of 20-3000pg/ml in both plasma and saliva using a 50µl sample volume. The partial validation sections outcome in saliva was so close to those in plasma. The within- and between-day precisions were all below 8.7% for plasma and below 11.4% for saliva. Accuracies ranged from 94 to 105% for both matrices. In this study, 26 healthy volunteers participated in the clinical study, and 6 of gave their saliva samples in addition to plasma at the same time schedule. The pharmacokinetic parameters of Cmax, AUC0-t and AUC0-∞, Tmax and T1/2 in both plasma and saliva were calculated and correlated. |
| File Format | HTM / HTML |
| ISSN | 15700232 |
| Volume Number | 972 |
| e-ISSN | 1873376X |
| Journal | Journal of Chromatography B |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-12-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Chemistry Antidiarrheals Metabolism Chromatography, High Pressure Liquid Methods Loperamide Saliva Tandem Mass Spectrometry Blood Humans Limit Of Detection Reproducibility Of Results Journal Article Validation Studies |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine Analytical Chemistry Clinical Biochemistry Biochemistry |
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