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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Van Schoors, Jolien Lens, Charlotte Maes, Katrien Michotte, Yvette Smolders, Ilse Van Eeckhaut, Ann |
| Description | Country affiliation: Belgium Author Affiliation: Van Schoors J ( Department of Pharmaceutical Chemistry and Drug Analysis, Center for Neurosciences, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Jette, Belgium.); Lens C ( Department of Pharmaceutical Chemistry and Drug Analysis, Center for Neurosciences, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Jette, Belgium.); Maes K ( Department of Pharmaceutical Chemistry and Drug Analysis, Center for Neurosciences, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Jette, Belgium.); Michotte Y ( Department of Pharmaceutical Chemistry and Drug Analysis, Center for Neurosciences, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Jette, Belgium.); Smolders I ( Department of Pharmaceutical Chemistry and Drug Analysis, Center for Neurosciences, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Jette, Belgium.); Van Eeckhaut A ( Department of Pharmaceutical Chemistry and Drug Analysis, Center for Neurosciences, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Jette, Belgium. Electronic address: aveeckha@vub.ac.be.) |
| Abstract | In recent years, the simultaneous monitoring of the monoamine neurotransmitters dopamine, noradrenaline and serotonin in vivo is advancing due to innovations in miniaturized and fast chromatographic techniques. However, the determination of the most hydrophilic compound, noradrenaline, in microdialysis samples by (ultra-)high performance liquid chromatography ((U)HPLC) with electrochemical detection (ECD) is impeded by a broad solvent front, caused by the addition of antioxidative agents. Hence, an elaborate reassessment of currently used antioxidative mixtures is necessary for further analytical improvements. The proposed mixture, containing 100mM acetic acid, 0.27mM Na2EDTA and 12.5µM ascorbic acid (pH 3.2), is less complex than previously described mixtures and shows minimal ECD interference. It stabilizes the three monoamines in standard solutions and in microdialysis samples, considering both autosampler stability at 4°C for 48h and long term stability at -20°C for a duration of six months. An in vivo microdialysis experiment demonstrates the possibility to monitor changes in extracellular levels of the three monoamines simultaneously in the rat hippocampus with UHPLC-ECD using the optimized antioxidative mixture. |
| File Format | HTM / HTML |
| ISSN | 15700232 |
| Volume Number | 998-999 |
| e-ISSN | 1873376X |
| Journal | Journal of Chromatography B |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2015-08-15 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Chemistry Antioxidants Chemistry Dopamine Neurotransmitter Agents Norepinephrine Serotonin Animals Chromatography, High Pressure Liquid Methods Drug Stability Hippocampus Male Microdialysis Rats Rats, Wistar Journal Article Research Support, Non-u.s. Gov't Validation Studies |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine Analytical Chemistry Clinical Biochemistry Biochemistry |
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