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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Zhang, Wei-min Li, Rui-fang Qiu, Jian-fei Zhang, Zhi-yin Wang, Hong-bo Bian, Lu Lei, Jia-hui |
| Description | Author Affiliation: Zhang WM ( The First Affiliated Hospital of Henan University of Science and Technology, Luoyang, Henan 471003, PR China. Electronic address: lyzhangweimin@163.com.); Li RF ( Medical College of Henan University of Science and Technology, Luoyang, Henan 471003, PR China.); Qiu JF ( Medical College of Henan University of Science and Technology, Luoyang, Henan 471003, PR China.); Zhang ZY ( Medical College of Henan University of Science and Technology, Luoyang, Henan 471003, PR China.); Wang HB ( Medical College of Henan University of Science and Technology, Luoyang, Henan 471003, PR China.); Bian L ( Medical College of Henan University of Science and Technology, Luoyang, Henan 471003, PR China.); Lei JH ( Medical College of Henan University of Science and Technology, Luoyang, Henan 471003, PR China.) |
| Abstract | A sensitive and rapid ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was developed to determine kurarinone in rat plasma using chlorzoxazone as the internal standard (IS). Sample preparation was accomplished through a liquid-liquid extraction procedure with ethyl acetate to 0.2 mL plasma sample. The analyte and IS were separated on an Acquity UPLC BEH C18 column (2.1 mm × 50 mm, 1.7 µm) with the mobile phase of acetonitrile and 1% formic acid in water with gradient elution at a flow rate of 0.40 mL/min. The detection was performed on a triple quadrupole tandem mass spectrometer equipped with electrospray ionization (ESI) by multiple reactions monitoring (MRM) of the transitions at m/z 437.0â301.2 for kurarinone and m/z 168.1â132.1 for IS. The linearity of this method was found to be within the concentration range of 20-2000 ng/mL with a lower limit of quantification of 20 ng/mL. Only 3.0 min was needed for an analytical run. The matrix effect was 94.7-107.2% for kurarinone. The intra- and inter-day precision (RSD%) were less than 8.2% and accuracy (RE%) was within ±9.0%. The recovery ranged from 77.3% to 85.6%. Kurarinone was sufficiently stable under all relevant analytical conditions. The method was also successfully applied to the pharmacokinetic study of kurarinone in rats. |
| File Format | HTM / HTML |
| ISSN | 15700232 |
| Volume Number | 986-987 |
| e-ISSN | 1873376X |
| Journal | Journal of Chromatography B |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2015-04-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Chemistry Chromatography, High Pressure Liquid Methods Flavonoids Blood Tandem Mass Spectrometry Administration, Intravenous Animals Drug Stability Administration & Dosage Chemistry Pharmacokinetics Linear Models Male Rats Rats, Sprague-dawley Reproducibility Of Results Sensitivity And Specificity Journal Article |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine Analytical Chemistry Clinical Biochemistry Biochemistry |
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