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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Tan, Suryani Rupasinghe, Thusitha W. T. Tull, Dedreia L. Augustin, Mary Ann Gras, Sally L. |
| Description | Author Affiliation: Tan S ( CSIRO Food, Nutrition and Bioproducts Flagship, 671 Sneydes Road, Werribee, Vic 3030, Australia); Rupasinghe TW ( Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, Vic 3010, Australia); Tull DL ( Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, Vic 3010, Australia); Augustin MA ( CSIRO Food, Nutrition and Bioproducts Flagship, 671 Sneydes Road, Werribee, Vic 3030, Australia.); Gras SL ( Department of Chemical and Biomolecular Engineering, The University of Melbourne, Parkville, Vic 3010, Australia) |
| Abstract | Liquid chromatography-mass spectrometry (LC-MS) has been used to detect polyphenolic curcuminoids found in turmeric but studies of metabolism by bacterial and mammalian cells in vitro are compromised by poor recovery from the culture medium. We report a liquid-liquid extraction procedure with ethyl acetate and use LC-MS to quantify extracted curcuminoids. Ethyl acetate allows recoveries of â¼ 80-86% of curcuminoids from the bacterial growth medium, bacterial cell lysate and combined bacterial cell and growth medium matrices; a clear improvement over acetonitrile where recoveries were â¼ 25-66%. This optimised method will enable studies of curcuminoid metabolism and may be applicable to other hydrophobic polyphenolic compounds. |
| File Format | HTM / HTML |
| ISSN | 15700232 |
| Volume Number | 988 |
| e-ISSN | 1873376X |
| Journal | Journal of Chromatography B |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2015-04-15 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Chemistry Chromatography, Liquid Methods Curcumin Isolation & Purification Escherichia Metabolism Liquid-liquid Extraction Mass Spectrometry Polyphenols Bioreactors Microbiology Analysis Linear Models Journal Article |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine Analytical Chemistry Clinical Biochemistry Biochemistry |
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