| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Gao, Qianqian Xu, Zisheng Zhao, Genhua Wang, Heng Weng, Zebin Pei, Ke Wu, Li Cai, Baochang Chen, Zhipeng Li, Weidong |
| Description |
Author Affiliation: Gao Q ( Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210023, China); Xu Z ( Wuhu Pure Sunshine Natural Medicine Company Limited, Wuhu 241000, China.); Zhao G ( Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210023, China); Wang H ( Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210023, China); Weng Z ( Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210023, China); Pei K ( Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210023, China); Wu L ( Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210023, China); Cai B ( Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210023, China); Chen Z ( Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210023, China); Li W ( Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210023, China) |
| Abstract |
Psoralea corylifolia L. has long been used in traditional Chinese medicine for treating and preventing many diseases. A group of flavonoid components are regarded as the active principals within the seeds. In this research, a rapid, accurate and sensitive ultra high pressure liquid chromatography tandem mass spectrometry (UHPLC/MS/MS) method has been established for simultaneous quantification of its 5 main components, namely, neobavaisoflavone, bavachin, isobavachalcone, bavachinin and corylifol A in rats' plasma after the rats were orally administrated with Buguzhi extract. Negative ion electrospray mode was applied in the detection process. Multiple reactions monitoring (MRM) mode was utilized for simultaneous quantitative analyzing of neobavaisoflavone (m/z 321.1âm/z 265.1), bavachin (m/z 323.1âm/z 119.0), isobavachalcone (m/z 323.2âm/z 119.0), bavachinin (m/z 337.2âm/z 119.0), corylifol A (m/z 389.2âm/z 277.0) and liquiritigenin (Internal Standard, m/z 255.1âm/z 119.0). Chromatographic separation of the above mentioned components was conducted on a Waters BEH-C18 column (100 mm×2.1mm, 1.7µm) with gradient elution system at flow rate of 0.3mL/min. The mobile phase was composed of acetonitrile and 0.1% formic acid solution. The lower limit of quantification (LLOQ) for each of the above analytes was 0.5ng/mL. Each of the analytes exhibited good linearity within the concentration range of 0.5-100ng/mL. The method was fully validated for its selectivity, accuracy, precision, stability, matrix effect and extraction recovery. The validated method has been successfully applied for simultaneous determination of the 5 flavonoids in rat plasma for the first time. |
| File Format |
HTM / HTML |
| ISSN |
15700232 |
| Volume Number |
1011 |
| e-ISSN |
1873376X |
| Journal |
Journal of Chromatography B |
| Language |
English |
| Publisher |
Elsevier |
| Publisher Date |
2016-02-01 |
| Publisher Place |
Netherlands |
| Access Restriction |
One Nation One Subscription (ONOS) |
| Subject Keyword |
Discipline Analytical Chemistry Chromatography, High Pressure Liquid Methods Flavonoids Blood Plant Extracts Pharmacokinetics Psoralea Chemistry Tandem Mass Spectrometry Animals Drug Stability Linear Models Male Administration & Dosage Rats Rats, Sprague-dawley Reproducibility Of Results Sensitivity And Specificity Journal Article Research Support, Non-u.s. Gov't |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Cell Biology Medicine Analytical Chemistry Clinical Biochemistry Biochemistry |
