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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Bonaiuto, Emanuela Grancara, Silvia Martinis, Pamela Stringaro, Annarita Colone, Marisa Agostinelli, Enzo Macone, Alberto Stevanato, Roberto Vianello, Fabio Toninello, Antonio Di Paolo, Maria Luisa |
| Description | Country affiliation: Italy Author Affiliation: Bonaiuto E ( Department of Molecular Medicine, University of Padova, Via G. Colombo 3, 35131 Padova, Italy.); Grancara S ( Department of Biomedical Sciences, University of Padova, Via G. Colombo 3, 35131 Padova, Italy.); Martinis P ( Department of Biomedical Sciences, University of Padova, Via G. Colombo 3, 35131 Padova, Italy.); Stringaro A ( Department of Technology and Health, Italian Institute of Health, 00161 Roma, Italy.); Colone M ( Department of Technology and Health, Italian Institute of Health, 00161 Roma, Italy.); Agostinelli E ( Istituto Pasteur-Fondazione Cenci Bolognetti, Department of Biochemical Sciences 'A. Rossi Fanelli,' Sapienza University of Rome and Institute of Biology and Molecular Pathology, Italian Research Council, P.le Aldo Moro 5, 00185 Roma, Italy.); Macone A ( Istituto Pasteur-Fondazione Cenci Bolognetti, Department of Biochemical Sciences 'A. Rossi Fanelli,' Sapienza University of Rome and Institute of Biology and Molecular Pathology, Italian Research Council, P.le Aldo Moro 5, 00185 Roma, Italy.); Stevanato R ( Department of Molecular Science and Nanosystems, Università Ca' Foscari, Dorsoduro 2137, 30123 Venezia, Italy.); Vianello F ( Department of Comparative Biomedicine and Food Science, Polo Agripolis, Viale dell'Università 16, University of Padova, 35020 Legnaro, Italy); Toninello A ( Department of Biomedical Sciences, University of Padova, Via G. Colombo 3, 35131 Padova, Italy. Electronic address: antonio.toninello@unipd.it.); Di Paolo ML ( Department of Molecular Medicine, University of Padova, Via G. Colombo 3, 35131 Padova, Italy) |
| Abstract | The uptake of spermine into mammalian mitochondria indicated the need to identify its catabolic pathway in these organelles. Bovine liver mitochondria were therefore purified and their capacity for natural polyamine uptake was verified. A kinetic approach was then used to determine the presence of an MDL 72527-sensitive enzyme with spermine oxidase activity in the matrix of bovine liver mitochondria. Western blot analysis of mitochondrial fractions and immunogold electron microscopy observations of purified mitochondria unequivocally confirmed the presence of a protein recognized by anti-spermine oxidase antibodies in the mitochondrial matrix. Preliminary kinetic characterization showed that spermine is the preferred substrate of this enzyme; lower activity was detected with spermidine and acetylated polyamines. Catalytic efficiency comparable to that of spermine was also found for 1-aminododecane. The considerable effect of ionic strength on the Vmax/KM ratio suggested the presence of more than one negatively charged zone inside the active site cavity of this mitochondrial enzyme, which is probably involved in the docking of positively charged substrates. These findings indicate that the bovine liver mitochondrial matrix contains an enzyme belonging to the spermine oxidase class. Because H2O2 is generated by spermine oxidase activity, the possible involvement of the latter as an important signaling transducer under both physiological and pathological conditions should be considered. |
| File Format | HTM / HTML |
| ISSN | 08915849 |
| Volume Number | 81 |
| e-ISSN | 18734596 |
| Journal | Free Radical Biology and Medicine |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2015-04-01 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Free radicals biology Liver Enzymology Mitochondria, Liver Oxidoreductases Acting On Ch-nh Group Donors Metabolism Spermine Animals Catalytic Domain Cattle Hydrogen Peroxide Kinetics Osmolar Concentration Oxidation-reduction Antagonists & Inhibitors Isolation & Purification Putrescine Analogs & Derivatives Chemistry Spermidine Static Electricity Substrate Specificity Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Physiology (medical) Biochemistry |
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