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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Gonçalves, Ana Maria D. Mendes, Sónia de Sanctis, Daniele Martins, Lígia O. Bento, Isabel |
| Description | Country affiliation: Portugal Author Affiliation: Gonçalves AM ( Macromolecular Crystallography Unit, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal.) |
| Abstract | The enzymatic degradation of azo dyes begins with the reduction of the azo bond. In this article, we report the crystal structures of the native azoreductase from Pseudomonas putida MET94 (PpAzoR) (1.60 Å), of PpAzoR in complex with anthraquinone-2-sulfonate (1.50 Å), and of PpAzoR in complex with Reactive Black 5 dye (1.90 Å). These structures reveal the residues and subtle changes that accompany substrate binding and release. Such changes highlight the fine control of access to the catalytic site that is required by the ping-pong mechanism, and in turn the specificity offered by the enzyme towards different substrates. The topology surrounding the active site shows novel features of substrate recognition and binding that help to explain and differentiate the substrate specificity observed among different bacterial azoreductases. |
| File Format | HTM / HTML |
| ISSN | 1742464X |
| Issue Number | 24 |
| Volume Number | 280 |
| e-ISSN | 17424658 |
| Journal | FEBS Journal |
| Language | English |
| Publisher | Wiley (on behalf of the Federation of European Biochemical Societies) |
| Publisher Date | 2013-12-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Biochemistry Azo Compounds Metabolism Nadh, Nadph Oxidoreductases Chemistry Pseudomonas Putida Enzymology Amino Acid Sequence Catalysis Catalytic Domain Crystallization Crystallography, X-ray Flavin Mononucleotide Kinetics Molecular Sequence Data Protein Conformation Substrate Specificity Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry Molecular Biology |
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