NDLI: The ascidian Styela plicata hemocytes as a potential biomarker of marine pollution: In vitro effects of seawater and organic mercury.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Parrinello, D. Bellante, A. Parisi, M. G. Sanfratello, M. A. Indelicato, S. Piazzese, D. Cammarata, M.
Description	Country affiliation: Italy Author Affiliation: Parrinello D ( Marine Immunobiology Laboratory, Dipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche University of Palermo, Palermo, Italy.); Bellante A ( Dipartimento di Scienze della Terra e del Mare, University of Palermo, Palermo, Italy.); Parisi MG ( Marine Immunobiology Laboratory, Dipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche University of Palermo, Palermo, Italy.); Sanfratello MA ( Marine Immunobiology Laboratory, Dipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche University of Palermo, Palermo, Italy.); Indelicato S ( Dipartimento di Scienze della Terra e del Mare, University of Palermo, Palermo, Italy.); Piazzese D ( Dipartimento di Scienze della Terra e del Mare, University of Palermo, Palermo, Italy.); Cammarata M ( Marine Immunobiology Laboratory, Dipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche University of Palermo, Palermo, Italy. Electronic address: matteo.cammarata@unipa.it.)
Abstract	Toxic metals, such as mercury, contribute substantially to anthropogenic pollution in many estuarine environments. Animals living in those environments, particularly invertebrate filter feeders like tunicates, can be used as bioindicators. In an attempt to identify cellular markers for revealing pollution, this study examined in vitro the effects of different concentrations of methyl mercury on Styela plicata hemocytes. The harvested hemocytes from S. plicata that were exposed to the metal had a significant mortality, cellular count and morphometric alterations. These findings provided evidence of MeHg immunotoxic effects on S. plicata, resulting in hemocyte death and morphological changes induced by cytoskeleton alterations. Thus, a morphometric cellular parameter, such as spreading ability, was used as a complementary method for differentiation between hemocytes treated with a marine solution (as a negative control) and hemocytes incubated with methylmercury and/or Sicilian seawater samples.
File Format	HTM / HTML
ISSN	01476513
Volume Number	136
e-ISSN	10902414
Journal	Ecotoxicology and Environmental Safety
Language	English
Publisher	Elsevier
Publisher Date	2017-02-01
Publisher Place	Netherlands
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Environmental Sciences Hemocytes Drug Effects Immunotoxins Toxicity Methylmercury Compounds Urochordata Animals Biological Markers Analysis Ultrastructure Microscopy, Electron, Scanning Journal Article
Content Type	Text
Resource Type	Article
Subject	Pollution Health, Toxicology and Mutagenesis Public Health, Environmental and Occupational Health

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