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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Huang, Chenrong Yang, Jian Du, Yonghong Miao, Liyan |
| Description | Country affiliation: China Author Affiliation: Huang C ( Clinical Pharmacology Research Laboratory, First Affiliated Hospital of Soochow University, Suzhou 215006, China.) |
| Abstract | BACKGROUND: A novel method has been developed for the determination of free warfarin in human plasma by ultra performance liquid chromatography combined with tandem mass spectrometry (UPLC-MS/MS). METHODS: The plasma filtrate was prepared by a high-speed ultrafiltration (UF), and was then extracted by a liquid/liquid extraction (LLE) with ethyl acetate. The chromatographic separation was performed on an Acquity UPLC BEH C(18) (2.1 mm x 50 mm, 1.7 microm, Waters) with a mobile phase consisting of 0.1% formic acid/acetonitrile (50:50, v/v). The analyses were carried out by multiple reaction monitoring (MRM) using the precursor-to-product combinations of m/z 307.3-->161.2 for warfarin and m/z 347.2-->161.3 for Cl-War. RESULTS: The lower limit of quantification (LLOQ) was 0.25 ng/ml and the assay exhibited a linear range of 0.25-16 ng/ml and gave a correlation coefficient (r) of > or = 0.9999. Quality control samples (0.5, 2, 8 ng/ml) in 5 replicates from 3 different runs of analysis demonstrated intra-assay CVs of 3.0-10.2%, inter-assay CVs of 4.2-12.0%, and an overall accuracy of 85-115%. CONCLUSIONS: The method can be applied to analyze the correlation of free concentration of warfarin and the international normalized ratio (INR). |
| File Format | HTM / HTML |
| ISSN | 00098981 |
| Issue Number | 2 |
| Volume Number | 393 |
| e-ISSN | 18733492 |
| Journal | Clinica Chimica Acta |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2008-07-17 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Clinical Chemistry Discipline Laboratory Medicine Blood Proteins Metabolism Chromatography, Liquid Methods International Normalized Ratio Tandem Mass Spectrometry Warfarin Blood Calibration Humans Reference Standards Reproducibility Of Results Sensitivity And Specificity Spectrometry, Mass, Electrospray Ionization Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry (medical) Clinical Biochemistry Biochemistry |
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