NDLI: Enzymatic synthesis of amoxicillin via a one-pot enzymatic hydrolysis and condensation cascade process in the presence of organic co-solvents.

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Enzymatic synthesis of amoxicillin via a one-pot enzymatic hydrolysis and condensation cascade process in the presence of organic co-solvents.

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Enzymatic synthesis of amoxicillin via a one-pot enzymatic hydrolysis and condensation cascade process in the presence of organic co-solvents.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Wu, Qi Chen, Chun-Xiu Du, Li-Li Lin, Xian-Fu
Description	Author Affiliation: Wu Q ( Department of Chemistry, Zhejiang University, Hangzhou 310027, People's Republic of China.)
Abstract	A cascade reaction combining the enzymatic hydrolysis of Penicillin G potassium salt (PGK) with the kinetically controlled enzymatic coupling of in situ formed 6-aminopenicillanic acid (6-APA) with p-hydroxyphenylglycine methyl ester (D-HPGM) to give amoxicillin as the final product by using a single enzyme has been demonstrated successfully. Ethylene glycol (EG) was employed as a component of reaction buffer to improve the synthesis yield. Reaction parameters, including different cosolvents, EG content, the loading of immobilized penicillin G acylase (IPA), and reaction temperature and time were studied to evaluate their effects on the reaction. The best result of 55.2% yield was obtained from the reaction which was carried out in the mixed media containing 40% sodium dihydrogen phosphate buffer (apparent pH 6.0) and 60% EG (v/v), with the initial concentration 150 mM and 450 mM of PGK and D-HPGM, respectively, catalyzed by 50 IU/mL IPA at 25 degrees C for 10 h. The IPA could be recycled for nine batches without obviously losing of catalytic activity. The important strategy will have potential application in the beta-lactam antibiotics industry due to the advantages of saving the effort of isolating 6-APA, reducing usual enzymatic steps and the industrial cost of amoxicillin synthesis.
File Format	HTM / HTML
ISSN	02732289
Issue Number	7
Volume Number	160
e-ISSN	15590291
Journal	Applied Biochemistry and Biotechnology
Language	English
Publisher	Springer
Publisher Date	2010-04-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Biochemistry Discipline Biotechnology Amoxicillin Chemical Synthesis Ethylene Glycol Chemistry Glycine Analogs & Derivatives Penicillin Amidase Penicillin G Phosphoric Acids Biocatalysis Enzymes, Immobilized Metabolism Hydrolysis Solvents Temperature Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Environmental Engineering Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology

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Enzymatic Synthesis of Amoxicillin via a One-pot Enzymatic Hydrolysis and Condensation Cascade Process in the Presence of Organic Co-solvents

Enhanced production of 6-aminopenicillanic acid in aqueous methyl isobutyl ketone system with immobilized penicillin G acylase.

A process to produce penicillin G acylase by surface-adhesion fermentation using Mucor griseocyanus to obtain 6-aminopenicillanic acid by penicillin G hydrolysis.

Effect of internal diffusional restrictions on the hydrolysis of penicillin G: reactor performance and specific productivity of 6-APA with immobilized penicillin acylase.

Effect of bed configuration of immobilized biocatalysts on penicillin G hydrolysis efficiency

Production of Ethylene Glycol from Glycerol Using an In Vitro Enzymatic Cascade

Enhanced Enzymatic Synthesis of a Cephalosporin, Cefadroclor, in the Presence of Organic Co-solvents

Effect of Internal Diffusional Restrictions on the Hydrolysis of Penicillin G: Reactor Performance and Specific Productivity of 6-APA with Immobilized Penicillin Acylase

Immobilization of penicillin G acylase on macro-mesoporous silica spheres.

Enzymatic synthesis of amoxicillin via a one-pot enzymatic hydrolysis and condensation cascade process in the presence of organic co-solvents.

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