NDLI: Exceptional stability of artemin neurotrophic factor dimers: effects of temperature, pH, buffer and storage conditions on protein integrity and activity.

Please wait, while we are loading the content...

Expression and secretion of the human erythropoietin using an optimized cbh1 promoter and the native CBH I signal sequence in the industrial fungus Trichoderma reesei.

Fermentation of Korean red ginseng by Lactobacillus plantarum M-2 and its immunological activities.

Nanotechnology: emerging tool for diagnostics and therapeutics.

Specificity of carboxypeptidases from Actinomucor elegans and their debittering effect on soybean protein hydrolysates.

Analysis of differentially expressed proteins in colorectal cancer using hydroxyapatite column and SDS-PAGE.

Expression of MMP2 and MMP9 (gelatinases A and B) in human colon cancer cells.

Cloning and Characterization of a Heme Oxygenase-2 Gene from Alfalfa (Medicago sativa L.)

Surface interactions and fouling properties of Micrococcus luteus with microfiltration membranes.

Optimization of a multiplex PCR assay for detecting transgenic soybean components in feed products.

Cloning and Sequence Analysis of Novel DNA Polymerases from Thermophilic Geobacillus Species Isolated from Hot Springs in Turkey: Characterization of a DNA Polymerase I from Geobacillus kaue Strain NB

New urea biosensor based on urease enzyme obtained from Helycobacter pylori.

Assessment of genetic stability and instability of tissue culture-propagated plantlets of Aloe vera L. by RAPD and ISSR markers.

Immobilization of Pseudomonas stutzeri lipase for the transesterification of wood sterols with fatty acid esters.

Isolation of phlorotannins from Eisenia bicyclis and their hepatoprotective effect against oxidative stress induced by tert-butyl hyperoxide.

Exceptional stability of artemin neurotrophic factor dimers: effects of temperature, pH, buffer and storage conditions on protein integrity and activity.

Direct and indirect organogenesis of Alpinia galanga and the phytochemical analysis.

Enzymatic Treatment and Detoxification of Acid Orange 7 from Textile Wastewater

Purification and properties of a new thermostable cyclodextrin glucanotransferase from Bacillus pseudalcaliphilus 8SB.

Characterization and identification of a chymotryptic hydrolysate of alpha-lactalbumin stimulating cholecystokinin release in STC-1 cells.

Purification and Characterization of the Enzyme Cholesterol Oxidase from a New Isolate of Streptomyces sp.

Production, purification, and biochemical characterization of a fibrinolytic enzyme from thermophilic Streptomyces sp. MCMB-379.

Inhibition effects of dilute-acid prehydrolysate of corn stover on enzymatic hydrolysis of Solka Floc.

Elicitor-induced cellular and molecular events are responsible for productivity enhancement in hairy root cultures: an insight study.

The application of ultrasound in the enzymatic hydrolysis of switchgrass.

Resveratrol content and expression of phenylalanine ammonia-lyase and stilbene synthase genes in cell cultures of Vitis amurensis treated with coumaric acid.

Year: 2011 Volume: 165 Issue: 7-8

Year: 2011 Volume: 164

Year: 2011 Volume: 163

Year: 2010 Volume: 162

Year: 2010 Volume: 161

Year: 2010 Volume: 160

Year: 2009 Volume: 159

Year: 2009 Volume: 158

Year: 2009 Volume: 156

Year: 2009 Volume: 155

Year: 2009 Volume: 154

Year: 2009 Volume: 153

Year: 2008 Volume: 151

Year: 2008 Volume: 150

Year: 2008 Volume: 149

Year: 2008 Volume: 148

Year: 2008 Volume: 146

Year: 2008 Volume: 145

Year: 2008 Volume: 144

Year: 2007 Volume: 137-140

Year: 2006 Volume: 131

Year: 1982 Volume: 7

Year: 1981 Volume: 6

Exceptional stability of artemin neurotrophic factor dimers: effects of temperature, pH, buffer and storage conditions on protein integrity and activity.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Bruinzeel, Wouter Masure, Stefan
Description	Country affiliation: Belgium Author Affiliation: Bruinzeel W ( Department of Assay Development & Target Validation, C.R.E.A.Te, Janssen Research & Development, Beerse, Belgium.)
Abstract	Artemin (ARTN) is a neurotrophic growth factor of the GDNF ligand family that signals through the specific GFR -3 coreceptor/cRet tyrosine kinase-mediated signaling cascade. Its expression and signaling action in adults are restricted to nociceptive sensory neurons in the dorsal root ganglia. Consequently, Artemin supports survival and growth of sensory neurons and has been studied as a possible treatment for neuropathic pain. We have developed a robust and sensitive cellular assay to measure ARTN biological activity. Using recombinant Artemin produced in Escherichia coli bacteria together with this specific assay, we demonstrate that ARTN is an exceptionally stable polypeptide. Multiple freeze-thaw cycles, incubation at elevated temperatures (up to 90 °C) for 0.5 h, prolonged storage at 4 °C, and exposure to conditions of different pH, salt concentration, and additives had no measurable effect on the biological activity of ARTN. In some of the tested conditions, partial removal of nine NH(2)-terminal amino acids of the ARTN protein occurred, but this truncation had no important effect on the ARTN signaling response. Consequently, we postulate that formulation and storage for in vivo testing of ARTN in neuropathic pain paradigms in animals and humans should be straightforward.
File Format	HTM / HTML
ISSN	02732289
Issue Number	5-6
Volume Number	165
e-ISSN	15590291
Journal	Applied Biochemistry and Biotechnology
Language	English
Publisher	Springer
Publisher Date	2011-11-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Biochemistry Discipline Biotechnology Nerve Tissue Proteins Chemistry Pharmacology Animals Biological Assay Cell Line Dimerization Drug Stability Humans Hydrogen-ion Concentration Mice Genetics Metabolism Protein Stability Temperature Journal Article
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Environmental Engineering Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Exceptional Stability of Artemin Neurotrophic Factor Dimers: Effects of Temperature, pH, Buffer and Storage Conditions on Protein Integrity and Activity

pH effects on the stability and dimerization of procaspase-3

Brown Kidney Bean Bowman-Birk Trypsin Inhibitor is Heat and pH Stable and Exhibits Anti-proliferative Activity.

Influences of acidic conditions on formazan assay: a cautionary note.

Glucose oxidase-dextran conjugates with enhanced stabilities against temperature and pH.

Structural and physical properties of collagen extracted from moon jellyfish under neutral pH conditions.

Engineering of protease-resistant phytase from Penicillium sp.: high thermal stability, low optimal temperature and pH.

Replacement P212H Altered the pH–Temperature Profile of Phytase from Aspergillus niger NII 08121

Molecular cloning, characterization, and dye-decolorizing ability of a temperature- and pH-stable laccase from Bacillus subtilis X1.

Exceptional stability of artemin neurotrophic factor dimers: effects of temperature, pH, buffer and storage conditions on protein integrity and activity.

Similar Documents

Exceptional Stability of Artemin Neurotrophic Factor Dimers: Effects of Temperature, pH, Buffer and Storage Conditions on Protein Integrity and Activity

pH effects on the stability and dimerization of procaspase-3

Brown Kidney Bean Bowman-Birk Trypsin Inhibitor is Heat and pH Stable and Exhibits Anti-proliferative Activity.

Influences of acidic conditions on formazan assay: a cautionary note.

Glucose oxidase-dextran conjugates with enhanced stabilities against temperature and pH.

Structural and physical properties of collagen extracted from moon jellyfish under neutral pH conditions.

Engineering of protease-resistant phytase from Penicillium sp.: high thermal stability, low optimal temperature and pH.

Replacement P212H Altered the pH–Temperature Profile of Phytase from Aspergillus niger NII 08121

Molecular cloning, characterization, and dye-decolorizing ability of a temperature- and pH-stable laccase from Bacillus subtilis X1.

Exceptional stability of artemin neurotrophic factor dimers: effects of temperature, pH, buffer and storage conditions on protein integrity and activity.