Please wait, while we are loading the content...
Please wait, while we are loading the content...
| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Tesfai, Berhane Teklehaimanot Wu, Dan Chen, Sheng Chen, Jian Wu, Jing |
| Description | Author Affiliation: Tesfai BT ( State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China.) |
| Abstract | Cyclodextrin glycosyltransferase (CGTase) is an enzyme that produces cyclodextrins from starch by an intramolecular transglycosylation reaction. Due to the increasing industrial application of cyclodextrins in many fields such as pharmacy, agriculture, biotechnology, food, environment and cosmetics, CGTases have attracted the attention of many scientific researches. Undoubtedly, due to its well-known genetic properties, simplicity and capacity to accommodate many foreign proteins, Escherichia coli remains the most widely used host for recombinant proteins production and thus for CGTases. Like all other proteins, CGTases are originally produced in the cytoplasm, but expressing them out into the periplasm or further to the culture media is preferred due to several advantages such as simplified downstream processing and high expression level which otherwise would be costly, complicated and time consuming. Since E. coli, other than some of its degradative enzymes and toxins, does not normally secrete proteins extracellularly, many strategies have been tried to overcome this drawback using the recombinant technologies. Unfortunately, oversecretion of the recombinant proteins most of the time results in the formation of inactive protein aggregates, called inclusion bodies, which result as a consequence of the burden caused by the methods meant to enhance the secretion. Thus, in this mini-review, the few but most commonly used strategies which offered a solution to the enhancement of extracellular secretion of CGTase in its native state are discussed. |
| File Format | HTM / HTML |
| ISSN | 02732289 |
| Issue Number | 4 |
| Volume Number | 167 |
| e-ISSN | 15590291 |
| Journal | Applied Biochemistry and Biotechnology |
| Language | English |
| Publisher | Springer |
| Publisher Date | 2012-06-01 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Biochemistry Discipline Biotechnology Escherichia Coli Genetics Extracellular Space Metabolism Genetic Engineering Methods Glucosyltransferases Biosynthesis Secretion Recombinant Proteins Culture Techniques Cytology Growth & Development Enzymology Journal Article Research Support, Non-u.s. Gov't Review |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Molecular Biology Environmental Engineering Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology |
National Digital Library of India (NDLI) is a virtual repository of learning resources which is not just a repository with search/browse facilities but provides a host of services for the learner community. It is sponsored and mentored by Ministry of Education, Government of India, through its National Mission on Education through Information and Communication Technology (NMEICT). Filtered and federated searching is employed to facilitate focused searching so that learners can find the right resource with least effort and in minimum time. NDLI provides user group-specific services such as Examination Preparatory for School and College students and job aspirants. Services for Researchers and general learners are also provided. NDLI is designed to hold content of any language and provides interface support for 10 most widely used Indian languages. It is built to provide support for all academic levels including researchers and life-long learners, all disciplines, all popular forms of access devices and differently-abled learners. It is designed to enable people to learn and prepare from best practices from all over the world and to facilitate researchers to perform inter-linked exploration from multiple sources. It is developed, operated and maintained from Indian Institute of Technology Kharagpur.
Learn more about this project from here.
NDLI is a conglomeration of freely available or institutionally contributed or donated or publisher managed contents. Almost all these contents are hosted and accessed from respective sources. The responsibility for authenticity, relevance, completeness, accuracy, reliability and suitability of these contents rests with the respective organization and NDLI has no responsibility or liability for these. Every effort is made to keep the NDLI portal up and running smoothly unless there are some unavoidable technical issues.
Ministry of Education, through its National Mission on Education through Information and Communication Technology (NMEICT), has sponsored and funded the National Digital Library of India (NDLI) project.
| Sl. | Authority | Responsibilities | Communication Details |
|---|---|---|---|
| 1 | Ministry of Education (GoI), Department of Higher Education |
Sanctioning Authority | https://www.education.gov.in/ict-initiatives |
| 2 | Indian Institute of Technology Kharagpur | Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project | https://www.iitkgp.ac.in |
| 3 | National Digital Library of India Office, Indian Institute of Technology Kharagpur | The administrative and infrastructural headquarters of the project | Dr. B. Sutradhar bsutra@ndl.gov.in |
| 4 | Project PI / Joint PI | Principal Investigator and Joint Principal Investigators of the project |
Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon |
| 5 | Website/Portal (Helpdesk) | Queries regarding NDLI and its services | support@ndl.gov.in |
| 6 | Contents and Copyright Issues | Queries related to content curation and copyright issues | content@ndl.gov.in |
| 7 | National Digital Library of India Club (NDLI Club) | Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach | clubsupport@ndl.gov.in |
| 8 | Digital Preservation Centre (DPC) | Assistance with digitizing and archiving copyright-free printed books | dpc@ndl.gov.in |
| 9 | IDR Setup or Support | Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops | idr@ndl.gov.in |
|
Loading...
|